DNA binding domain of RFX5: interactions with X-box DNA and RFXANK

Biochimica Et Biophysica Acta
Madhumita ChakrabortyAbhijit Chakrabarti

Abstract

Regulatory factor X (RFX) is a heterotrimeric protein complex having RFX5, RFXANK and RFXAP as its three subunits. It is involved in the regulation of the transcription of MHCII molecules in antigen presenting cells. The RFX complex binds to X-box DNA, using the DNA binding domain, present in RFX5. The DNA binding domain (DBD) of RFX5 (12kD) and intact RFXANK (35 kD) were subcloned, expressed and purified. The associations of RFX5DBD with the X-box DNA and between RFX5DBD and RFXANK were measured in this study. The interaction of RFX5DBD and X-box DNA was studied using steady state fluorescence quenching and circular dichroism. The binding dissociation constant (K(d)) of the DNA-protein complex was determined from fluorescence measurements. The van't Hoff plot was linear over the temperature range 10-25 degrees C and the binding was found to be entropy-driven and enthalpy-favorable. The effect of electrolytes in RFX5DBD-DNA association was also studied. Molecular association between RFX5DBD and RFXANK has been observed by fluorescence resonance energy transfer (FRET) measurements, changes in the ratio of the two vibronic intensities of pyrene labeled RFX5DBD in presence of RFXANK and chemical cross-linking followed by tandem ma...Continue Reading

References

Jan 1, 1978·Methods in Enzymology·R H Fairclough, C R Cantor
Nov 4, 1976·Journal of Molecular Biology·M T RecordP De Haseth
Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·Y TakedaC P Mudd
Jan 1, 1994·Annual Review of Immunology·P Cresswell
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B ForoodK P Nambiar
Jan 1, 1996·Annual Review of Immunology·B MachW Reith
Feb 1, 1997·Current Opinion in Immunology·J M Boss
Jun 20, 1998·Nucleic Acids Research·F CornilleW Reith
Aug 11, 2000·Molecular and Cellular Biology·A M DeSandroJ M Boss
Nov 30, 2000·Journal of the American Society of Nephrology : JASN·Katherine RaderMarilyn Gist Farquhar
Mar 13, 2001·Annual Review of Immunology·W Reith, B Mach
Oct 9, 2002·Nature Immunology·Nada NekrepB Matija Peterlin
May 10, 2003·Blood Cells, Molecules & Diseases·Poppy DattaAbhijit Chakrabarti
Sep 23, 2003·Nucleic Acids Research·Kausiki Datta, Vince J LiCata
Oct 20, 2004·The Journal of Biological Chemistry·Malyasri BhattacharyyaAbhijit Chakrabarti
May 12, 2010·Biophysical Chemistry·Madhumita ChakrabortyAbhijit Chakrabarti
Jun 1, 2007·Proteomics. Clinical Applications·Dipankar BhattacharyaAbhijit Chakrabarti

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