DNA binding, nuclease activity and cytotoxicity studies of Cu(II) complexes of tridentate ligands

Dalton Transactions : an International Journal of Inorganic Chemistry
Pankaj KumarDebasis Manna

Abstract

Cu(II) complexes of three tridentate ligands, L(1), L(2) and L(3), [L(1), N-((1H-imidazole-2-yl)methyl)-2-(pyridine-2-yl)ethanamine; L(2), N-((1-methyl-1H-imidazole-2-yl)methyl)-2-(pyridine-2-yl)ethanamine; L(3), 2-(pyridine-2-yl)-N-((pyridine-2-yl)methyl)ethanamine] respectively, were synthesized and characterized. The single crystal X-ray structure of complex 1 reveals the pseudo octahedral coordination geometry around the copper center. Absorption and fluorescence experimental evidence show good DNA binding propensity (in the order of 10(5) M(-1)) of the complexes. Thermal denaturation and circular dichroism (CD) analyses reveal minor structural changes of calf thymus (CT) DNA in presence of complexes and groove and/or surface binding of the complexes to CT-DNA. Kinetic DNA cleavage assay shows pseudo-first-order kinetic reaction between the complex and supercoiled (SC) DNA. In addition, mechanistic SC DNA cleavage results show higher DNA cleavage activity in presence of reducing agent, due to the presence of hydroxyl radicals. In vitro cytotoxicity assay of the complexes demonstrate that the complexes have low toxicity for different cancer cell lines and IC(50) values were between 37 and 156 μM.

References

Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J C FrançoisC Hélène
May 25, 2001·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·A Sreedhara, J A Cowan
Jun 8, 2001·Journal of the American Chemical Society·K J HumphreysS E Rokita
Jul 18, 2001·Journal of the American Chemical Society·M E BranumL Que
Dec 26, 2001·Chemical Reviews·M J ClarkeD R Frasca
Jul 4, 2002·Journal of the American Chemical Society·Kristi J HumphreysSteven E Rokita
Aug 31, 2002·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Kristi J HumphreysSteven E Rokita
Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jan Reedijk
Jul 24, 2003·Chemical Society Reviews·Clive Metcalfe, Jim A Thomas
Oct 1, 1961·Journal of Molecular Biology·J MARMUR, P DOTY
Mar 23, 2005·Dalton Transactions : an International Journal of Inorganic Chemistry·Konstantina KaridiJan Reedijk
May 19, 2005·Chemical Communications : Chem Comm·Fabrizio MancinUmberto Tonellato
Nov 9, 2007·Chemical Communications : Chem Comm·Brian M ZeglisJacqueline K Barton
Dec 17, 2008·Chemical Communications : Chem Comm·Ta-Sheng Andrew Tseng, Judith N Burstyn
Jan 13, 2010·Dalton Transactions : an International Journal of Inorganic Chemistry·Tereza AfratiDimitris P Kessissoglou
May 8, 2010·Dalton Transactions : an International Journal of Inorganic Chemistry·Amrita GhoshAmitava Das
Aug 4, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Pankaj KumarBiplab Mondal

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Citations

Oct 10, 2013·Chemical Reviews·Carlo SantiniCristina Marzano
Apr 26, 2014·European Journal of Medicinal Chemistry·Natarajan Raman, Narayanaperumal Pravin
Jul 6, 2014·Journal of Photochemistry and Photobiology. B, Biology·Muthusamy SelvaganapathyNatarajan Raman
Nov 9, 2015·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Sagiv WeintraubArie Gruzman
Oct 29, 2013·European Journal of Medicinal Chemistry·Subramaniyam RajalakshmiBalachandran Unni Nair
Jun 9, 2016·European Journal of Medicinal Chemistry·Saurav PaulDebasis Manna
Jan 7, 2017·Inorganic Chemistry·Alda Lisa ConciaA Jalila Simaan
Apr 29, 2015·Dalton Transactions : an International Journal of Inorganic Chemistry·Xue-Quan ZhouShi-Ping Yan
Mar 5, 2015·Dalton Transactions : an International Journal of Inorganic Chemistry· SaswatiRupam Dinda
Mar 25, 2016·Dalton Transactions : an International Journal of Inorganic Chemistry·Rakesh Kumar GuptaDaya Shankar Pandey
May 30, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Sartaj TabassumFarukh Arjmand
Nov 7, 2013·Acta Crystallographica. Section C, Crystal Structure Communications·Danielle L JacobsAbby R O'Connor
Jun 23, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Angelina PetrovićJovana Bogojeski
Apr 30, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Nathalie BerthetFabrice Thomas
May 23, 2014·Dalton Transactions : an International Journal of Inorganic Chemistry·Magdalena BarwiolekJulia Jezierska

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