DNA Binding Peptide Directed Synthesis of Continuous DNA Nanowires for Analysis of Large DNA Molecules by Scanning Electron Microscope

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Kyung-Il KimJung Heon Lee

Abstract

Synthesis of smooth and continuous DNA nanowires, preserving the original structure of native DNA, and allowing its analysis by scanning electron microscope (SEM), is demonstrated. Gold nanoparticles densely assembled on the DNA backbone via thiol-tagged DNA binding peptides work as seeds for metallization of DNA. This method allows whole analysis of DNA molecules with entangled 3D features.

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Related Concepts

Three-dimensional
Microscopes, Electron, Scanning
Sulfhydryl Compounds
DNA Binding
Structure
Polypeptides
Analysis
Gold
Vertebral Column
Nanowires

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