PMID: 3763392Sep 11, 1986Paper

DNA binding properties of a 110 kDa nucleolar protein

Nucleic Acids Research
M SappA Richter

Abstract

A single strand specific DNA binding protein was purified to homogeneity from calf thymus nucleoprotein. The monomeric protein is elongated in shape and has a molecular mass of 110 kDa. Since immunocytochemistry revealed that the protein is predominantly located in the nucleolus we refer to it as the 110 kDa nucleolar protein. The protein binds not only to single stranded DNA but also to single stranded RNA, including homopolymeric synthetic RNA. We have used the single stranded DNA binding properties of the 110 kDa protein in model studies to investigate its effects on the configuration of nucleic acid. Our results are: only 50-55 protein molecules are sufficient to saturate all binding sites on the 6408 nucleotides of phage fd DNA; protein binding cause a compaction of single stranded DNA; large nucleoprotein aggregates are formed in the presence of divalent cations; this is due to protein-protein interactions which occur at moderately high concentrations of magnesium-, calcium or manganese ions; the protein induces the reassociation of complementary nucleic acid sequences. We speculate that the 110 kDa protein performs similar reactions in vivo and may have a function related to the processing and packaging of preribosomal RNA.

References

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Citations

Apr 1, 1990·Chromosoma·P J DiMario, J G Gall
May 1, 1995·The Journal of Cell Biology·D Weisenberger, U Scheer
Jan 25, 1993·Nucleic Acids Research·P HemmerichU Krawinkel
Feb 15, 1989·European Journal of Biochemistry·M SappM O Olson
Jan 1, 1987·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·G ReimerU Scheer
Jan 26, 1999·Critical Reviews in Biochemistry and Molecular Biology·R Tuteja, N Tuteja
Mar 18, 2011·The FEBS Journal·Martina DoetschBoris Fürtig
Jun 14, 1991·Biochemical and Biophysical Research Communications·K Sipos, M O Olson
Nov 9, 2010·RNA Biology·Martina DoetschBoris Fürtig
Sep 17, 2014·Gene·Nathalie Durut, Julio Sáez-Vásquez
Nov 28, 1989·Biochemistry·T S DumbarM O Olson
Feb 17, 2017·Plant & Cell Physiology·Thanikarn UdomchalothornSupachitra Chadchawan
Jan 5, 2002·The Journal of Biological Chemistry·Benoit RogerPhilippe Bouvet
Aug 1, 1987·Molecular and Cellular Biology·A Y JongJ L Campbell

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