PMID: 9547272Jun 20, 1998Paper

DNA binding properties of a chemically synthesized DNA binding domain of hRFX1

Nucleic Acids Research
F CornilleW Reith

Abstract

The RFX DNA binding domain (DBD) is a novel highly conserved motif belonging to a large number of dimeric DNA binding proteins which have diverse regulatory functions in eukaryotic organisms, ranging from yeasts to human. To characterize this novel motif, solid phase synthesis of a 76mer polypeptide corresponding to the DBD of human hRFX1 (hRFX1/DBD), a prototypical member of the RFX family, has been optimized to yield large quantities (approximately 90 mg) of pure compound. Preliminary two-dimensional1H NMR experiments suggested the presence of helical regions in this sequence in agreement with previously reported secondary structure predictions. In gel mobility shift assays, this synthetic peptide was shown to bind in a cooperative manner the 23mer duplex oligodeoxynucleotide corresponding to the binding site of hRFX1, with a 2:1 stoichoimetry due to an inverse repeat present in the 23mer. The stoichiometry of this complex was reduced to 1:1 by decreasing the length of the DNA sequence to a 13mer oligonucleotide containing a single half-site. Surface plasmon resonance measurements were achieved using this 5'-biotylinated 13mer oligonucleotide immobilized on an avidin-coated sensor chip. Using this method an association consta...Continue Reading

References

Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J B Le PecqB Roques
Dec 1, 1994·Protein Science : a Publication of the Protein Society·E G Hutchinson, J M Thornton
Jan 1, 1996·Annual Review of Immunology·B MachW Reith
Oct 1, 1996·Nature Structural Biology·G ParkinsonR H Ebright
Jan 1, 1995·Journal of Peptide Science : an Official Publication of the European Peptide Society·T JohnsonR C Sheppard

❮ Previous
Next ❯

Citations

Dec 28, 1999·Journal of Molecular Recognition : JMR·D G Myszka
Mar 30, 2001·Journal of Peptide Science : an Official Publication of the European Peptide Society·B P Roques
Feb 19, 2000·Current Opinion in Structural Biology·K S Gajiwala, S K Burley
Jul 20, 2010·Biochimica Et Biophysica Acta·Madhumita ChakrabortyAbhijit Chakrabarti
Oct 27, 2009·Biophysical Chemistry·Elmar NurmemmedovMarjolein M G M Thunnissen
May 10, 2020·The Analyst·Xuelin JinKyubong Jo
Aug 15, 2002·American Journal of Pharmacogenomics : Genomics-related Research in Drug Development and Clinical Practice·R Gambari
Apr 6, 2001·Analytical Chemistry·F LisdatR Brigelius-Flohé
Apr 2, 2005·Journal of Medicinal Chemistry·Chi ZhangYaoqi Zhou

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.