PMID: 2502535Aug 1, 1989Paper

DNA-binding proteins in cells and membrane blebs of Neisseria gonorrhoeae

Journal of Bacteriology
D W Dorward, C F Garon

Abstract

Naturally elaborated membrane bleb fractions BI and BII of Neisseria gonorrhoeae contain both linear and circular DNAs. Because little is known about the interactions between DNA and blebs, studies were initiated to identify specific proteins that bind DNA in elaborated membrane blebs. Western immunoblots of whole-cell and bleb proteins from transformation-competent and DNA-uptake-deficient (dud) mutants were probed with single- or double-stranded gonococcal DNA, pBR322, or synthetic DNA oligomers containing intact or altered gonococcal transformation uptake sequences. The specificity and sensitivity of a nonradioactive DNA-binding protein assay was evaluated, and the assay was used to visualize DNA-protein complexes on the blots. The complexes were then characterized by molecular mass, DNA-binding specificity, and expression in bleb fractions. The assay effectively detected blotted DNA-binding proteins. At least 17 gonococcal DNA-binding proteins were identified; unique subsets occurred in BI and BII. Certain DNA-binding proteins had varied affinities for single- and double-stranded DNA, and the intact transformation uptake sequence competitively displaced the altered sequence from a BI protein at 11 kilodaltons (kDa). A dud m...Continue Reading

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Citations

Apr 8, 2016·Clinical and Translational Medicine·Björn L D M BrücherIjaz S Jamall
Jun 27, 2017·Frontiers in Microbiology·Arif Tasleem Jan
Nov 7, 2018·Nature Reviews. Microbiology·Masanori ToyofukuLeo Eberl
Jul 22, 1998·FEMS Microbiology Letters·L ZhouR J Doyle
Apr 25, 2000·The Journal of Biological Chemistry·A L Horstman, M J Kuehn
Mar 24, 2006·FEMS Microbiology Letters·Marcela O Ramón RochaErasmo Negrete-Abascal
May 23, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C PeterssonK E Magnusson
Jun 3, 2014·BMC Cancer·Björn Ldm Brücher, Ijaz S Jamall
Dec 15, 2010·Seminars in Immunopathology·Can M UnalKristian Riesbeck
Jul 18, 2018·Frontiers in Immunology·Lorinda TurnerMaria Kaparakis-Liaskos
Aug 6, 2008·Infection and Immunity·Stefan SchildAndrew Camilli
Feb 3, 2005·Journal of Bacteriology·Erin J van SchaikRandall T Irvin
Sep 30, 2000·Applied and Environmental Microbiology·S YaronK R Matthews
Jan 1, 1994·Progress in Biophysics and Molecular Biology·A G Sabelnikov
Mar 17, 2021·Molecular Immunology·William J GilmoreMaria Kaparakis-Liaskos
May 9, 2021·Applied Microbiology and Biotechnology·Tomás G VillaAngeles Sánchez-Pérez
Nov 1, 2005·EcoSal Plus·Amanda J McBroom, Meta J Kuehn
Jun 1, 1990·Applied and Environmental Microbiology·D W Dorward, C F Garon
Jun 25, 2015·Microbiology Spectrum·Kyle P Obergfell, H Steven Seifert
Nov 18, 2005·Genes & Development·Meta J Kuehn, Nicole C Kesty

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