PMID: 2510170Nov 1, 1989Paper

DNA binding site of the growth factor-inducible protein Zif268

Proceedings of the National Academy of Sciences of the United States of America
B Christy, D Nathans

Abstract

Zif268, a zinc finger protein whose mRNA is rapidly activated in cells exposed to growth factors or other signaling agents, is thought to play a role in regulating the genetic program induced by extracellular ligands. We report that Zif268 has one of the characteristics of a transcriptional regulator, namely, sequence-specific binding to DNA. Zif268 synthesized in Escherichia coli bound to two sites upstream of the zif268 gene and to sites in the promoter regions of other genes. The nucleotide sequences responsible for binding were defined by DNase I footprinting, by methylation interference experiments, and by use of synthetic oligonucleotides. From these results we derived the following consensus sequence for a Zif268 high-affinity binding site: GCGTGGGGCG.

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Citations

Apr 29, 1998·The Journal of Comparative Neurology·C V Mello, S Ribeiro
Mar 16, 2000·The Journal of Comparative Neurology·E D Jarvis, C V Mello
Feb 1, 1997·Journal of Neuroscience Research·L A WhiteS R Whittemore
Sep 15, 1997·Journal of Cellular Biochemistry·R P HuangE D Adamson
Nov 4, 2004·Journal of Cellular Biochemistry·Alia Al-SarrajGerald Thiel
Dec 1, 1994·Journal of Cellular Biochemistry·R P HuangE D Adamson
Oct 18, 2002·Journal of Cellular Physiology·Gerald Thiel, Giuseppe Cibelli
Feb 15, 1993·Journal of Neuroscience Research·S R MullerS C Feinstein
Jan 1, 1993·Molecular & General Genetics : MGG·V SophianopoulouC Scazzocchio
Jan 1, 1995·Archives of Dermatological Research·K Elgjo, K L Reichelt
Jan 1, 1991·Molecular Neurobiology·W C AbrahamW P Tate
May 3, 2011·Calcified Tissue International·Marie K ReumannPhilipp Mayer-Kuckuk
Sep 29, 2011·Pflügers Archiv : European journal of physiology·Sidarta Ribeiro
Dec 8, 2011·Breast Cancer Research and Treatment·Adele VivacquaMarcello Maggiolini
Jan 25, 1995·Biochimica Et Biophysica Acta·Y Takimoto, A Kuramoto
Jun 30, 1994·Biochimica Et Biophysica Acta·J Szeberényi, P Erhardt
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Jan 3, 1994·Neuroscience Letters·J HermsK H Schlingensiepen

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