DNA binding sites recognised in vitro by a knotted class 1 homeodomain protein encoded by the hooded gene, k, in barley (Hordeum vulgare)

FEBS Letters
L KrusellK Gausing

Abstract

The homeodomain of the knotted classes of transcription factors from plants differs from the well characterized Antp/En type homeodomains from Drosophila at key amino acid residues contributing to the DNA binding. A cDNA, Hvh21, derived from the hooded gene and encoding a full length homolog of knotted1 from maize was isolated from barley seedlings and expressed as a maltose binding protein fusion in E. coli. The purified HvH21-fusion protein selected DNA fragments with 1-3 copies of the sequence TGAC. Gel shift experiments showed that the TGAC element was required for binding and the results further indicate that the HvH21-fusion protein binds DNA as a monomer.

References

Dec 3, 1991·Biochemistry·A Laughon
Jul 1, 1986·European Journal of Biochemistry·K Gausing, R Barkardottir
Jan 1, 1994·Annual Review of Biochemistry·W J GehringT Bürglin
Sep 1, 1993·The Plant Cell·M MatsuokaY Kano-Murakami
Oct 1, 1993·European Journal of Biochemistry·P S Nielsen, K Gausing

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Citations

Jun 10, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R W Williams
Oct 10, 1998·Biochimica Et Biophysica Acta·R L ChanD H Gonzalez
Jul 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Harley M S SmithSarah Hake
Oct 29, 1999·DNA and Cell Biology·M L TejadaR G Deeley
Jun 21, 2011·Plant Physiology·Silvana V SpinelliJavier F Palatnik
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Sarah HakeJulio Ramirez
Sep 29, 2011·Journal of Integrative Plant Biology·Bi-Qing WenHong-Wei Xue
Sep 6, 2001·Genesis : the Journal of Genetics and Development·Y ItoN Kurata
Apr 14, 2004·The Plant Journal : for Cell and Molecular Biology·Hao ChenDavid J Hannapel
Aug 26, 2020·Proceedings of the National Academy of Sciences of the United States of America·Ying Hua SuXian Sheng Zhang

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