PMID: 8610101Apr 2, 1996Paper

DNA binding specificity of two homeodomain proteins in vitro and in Drosophila embryos

Proceedings of the National Academy of Sciences of the United States of America
J Walter, Mark D Biggin

Abstract

In previous experiments, the homeodomain proteins even-skipped and fushi-tarazu were found to UV cross-link to a surprisingly wide array of DNA sites in living Drosophila embryos. We now show that UV cross-linking gives a highly accurate measure of DNA binding by these proteins. In addition, the binding of even-skipped and fushi-tarazu proteins has been measured in vitro to the same DNA fragments that were examined in vivo. This analysis shows that these proteins have broad DNA recognition properties in vitro that are likely to be important determinants of their distribution on DNA in vivo, but it also shows that in vitro DNA binding specificity alone is not sufficient to explain the distribution of these proteins in embryos.

References

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Citations

Jun 23, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M Mannervik
Feb 1, 2003·Archives of Biochemistry and Biophysics·Jan Norberg
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·J D Laney, M D Biggin
Oct 6, 1999·Current Opinion in Genetics & Development·D Dorsett
Dec 21, 2002·Evolution & Development·Carl D Schlichting
Jun 29, 1999·The Journal of Cell Biology·T TumbarA S Belmont
Sep 25, 2010·Trends in Genetics : TIG·Robin D Dowell
Sep 1, 2004·Biochemical and Biophysical Research Communications·Lemin ZhangFritz Schöffl
Oct 13, 1998·Current Biology : CB·M Akam
Aug 14, 1999·Mechanisms of Development·C Maurel-ZaffranD Aragnol
Jun 4, 2010·PloS One·Stefanie D HueberTancred Frickey
Dec 15, 2006·Development·Stefanie D HueberIngrid Lohmann

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