DNA condensation by PAMAM dendrimers: self-assembly characteristics and effect on transcription

Biochemistry
Kristina FantBengt Nordén

Abstract

Electrostatic shielding and steric blocking by histones are two significant factors that participate in the control of the local rates of transcription in chromatin. As a simple model system to determine how the degree of DNA condensation affects enzyme accessibility and gene expression, we have used generation 5 polyamidoamine (G5 PAMAM) cationic dendrimer particles (size 5.4 nm) as a synthetic histone model together with an in vitro transcription assay. The degree of compaction, conformation, and binding availability of the dendrimer-DNA complexes is characterized by linear and circular dichroism, dynamic light scattering, and competitive binding of ethidium. Using ultracentrifugation we are able to show explicitly, for the first time, that dendrimer particles bind to DNA in a highly cooperative manner, and that the dendrimer-induced condensation of the DNA strongly attenuates transcription. Two fractions with different properties can be identified: a low-density fraction which behaves very similar to uncondensed DNA and a high-density fraction which is condensed to a high extent and where binding availability and transcription are strongly reduced. Circular dichroism gives clues to the structure of the condensed DNA indicati...Continue Reading

References

Feb 1, 1992·Quarterly Reviews of Biophysics·B NordenT Kurucsev
Oct 1, 1994·Biophysical Journal·J L SikoravF Livolant
Oct 18, 2001·International Journal of Pharmaceutics·M D BrownI F Uchegbu
Jan 8, 2003·The Journal of Gene Medicine·Andrei V MaksimenkoClaude Malvy
Dec 23, 2004·Journal of Pharmaceutical Sciences·Chad S BraunC Russell Middaugh
Feb 3, 2005·The European Physical Journal. E, Soft Matter·J DeRoucheyJ O Rädler
May 24, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Koki WadaKaneto Uekama
Dec 19, 2006·Nature Reviews. Genetics·Shiv I S Grewal, Songtao Jia

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Citations

Jul 4, 2012·Journal of Biosciences·Bidisha NandyPrabal K Maiti
May 7, 2013·Colloids and Surfaces. B, Biointerfaces·Elzbieta Pedziwiatr-WerbickaMaria Bryszewska
Jul 25, 2012·Molecular Pharmaceutics·Lisa E PrevetteMark M Banaszak Holl
May 29, 2014·Physical Chemistry Chemical Physics : PCCP·Khawla QamhiehMarie-Louise Ainalem
Jun 24, 2014·The Journal of Physical Chemistry. B·Marina V Zhiryakova, Vladimir A Izumrudov
Jul 9, 2013·International Journal of Pharmaceutics·Inessa HaletsMaria Bryszewska
Sep 11, 2014·The Journal of Physical Chemistry. B·Taraknath MandalPrabal K Maiti
Sep 6, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shi YuRonald G Larson
Sep 5, 2015·Journal of Drug Targeting·Mohammad HemmatiReza Shirkoohi
Apr 24, 2012·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·Yuya HayashiHidetoshi Arima
Jun 11, 2014·Journal of Inorganic Biochemistry·Fei-Fei BaoZhi-Guo Gu
Sep 8, 2011·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Hidetoshi ArimaKaneto Uekama
Jun 4, 2011·Bioorganic & Medicinal Chemistry·Shujiro ShiosakiYoshiki Katayama
Oct 10, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Dzmitry ShcharbinMaria Bryszewska
Apr 1, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Dzmitry ShcharbinMaria Bryszewska
Jan 6, 2009·Journal of Inorganic Biochemistry·Michelle J PisaniJ Grant Collins
Jun 10, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Daniel Edinger, Ernst Wagner
Oct 23, 2010·Chemistry : a European Journal·Xindian DongZijian Guo
Mar 19, 2013·Cell Biology International·Nasibeh DaneshvarParvaneh Mehrbod
May 25, 2010·Chemistry : a European Journal·Giovanni Maria PavanSabrina Pricl
Sep 24, 2013·Polymer·Mallory A van DongenMark M Banaszak Holl
Dec 31, 2010·Colloids and Surfaces. B, Biointerfaces·Elzbieta Pedziwiatr-WerbickaMaria Bryszewska
Jan 21, 2015·Colloids and Surfaces. B, Biointerfaces·Azahara Rata-AguilarAntonio Martín-Rodríguez
Dec 18, 2008·Physical Chemistry Chemical Physics : PCCP·Giacomo Martini, Laura Ciani
Nov 4, 2010·Physical Chemistry Chemical Physics : PCCP·Dan LundbergBjörn Lindman
Jul 5, 2017·Organic & Biomolecular Chemistry·H ZhouR Z Qiao
Sep 25, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Azadeh ReshadmaneshFarnoush Jafari Iri Sofla
Jun 5, 2020·Biomacromolecules·Chanel C LaJayachandran N Kizhakkedathu

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