DNA content and alkaline phosphatase expression in cells of different gingival overgrowths

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
L T Hou, J A Yaeger

Abstract

The present study compared the alkaline phosphatase (ALPase) expression and DNA content at specific periods in cultured cells derived from non-inflamed enlarged gingivae of idiopathic gingivofibromatosis (IGF) and phenytoin-induced hyperplasia (PHG). Cultured cells from healthy gingiva or periodontal ligament (PDL) were used as controls. The DNA assay, ALPase assay and cytochemical staining for ALPase in cultured cells were performed at four, seven, and nine days. The presence of intense ALPase activity was a prominent feature in cultured IGF cells, whereas very low ALPase activity was detected in PHG cells. The cell lines tested showed no significant differences in DNA content. The expression of ALPase in these cells was population density-dependent. The observation that cells isolated from both types of gingival overgrowth exhibited a different ALPase profile at variance with normal gingival fibroblasts suggested that a distinct pathogenic mechanism may be involved in each type of gingival overgrowth.

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Citations

Aug 26, 2000·Journal of Periodontology·L T HouJ K Chen
Apr 13, 2007·Macromolecular Bioscience·Ming-Hua HoJuin-Yih Lai
Feb 14, 2013·The Journal of Contemporary Dental Practice·Sunkara Shree Ramalinga PrasadSv Kiran Kumar
Sep 2, 1999·Archives of Oral Biology·L T HouH F Thomas

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