DNA content in Acanthamoeba during two stress defense reactions: Encystation, pseudocyst formation and cell cycle.

European Journal of Protistology
Eva BínováEva Nohýnková

Abstract

During environmental stress, the vegetative cells of the facultative pathogenic amoeba Acanthamoeba castellanii reversibly differentiate into resistant dormant stages, namely, cysts or pseudocysts. The type of resistant stage depends on the nature and duration of the stressor. Cell differentiation is accompanied by changes in morphology and cellular metabolism. Moreover, cell differentiation is also expected to be closely linked to the regulation of the cell cycle and, thus, to cellular DNA content. While the existence of the resistant stages in A. castellanii is well known, there is no consensus regarding the relationship between differentiation and cell cycle progression. In the present work, we used flow cytometry analysis to explore the changes in the DNA content during Acanthamoeba encystation and pseudocyst formation. Our results strongly indicate that A. castellanii enters encystation from the G2 phase of the cell cycle. In contrast, differentiation into pseudocysts can begin in the G1 and G2 phases. In addition, we present a phylogenetic analysis and classification of the main cell cycle regulators, namely, cyclin-dependent kinases and cyclins that are found in the genome of A. castellanii.

References

Jan 1, 1976·Annual Review of Microbiology·R A Weisman
Mar 1, 1991·Reviews of Infectious Diseases·T J ByersE R Hugo
Mar 1, 1990·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·R E EdelsonT L MacDonald
Sep 1, 1973·Antimicrobial Agents and Chemotherapy·R A BucknallB Hesp
Nov 1, 1973·The Journal of protozoology·R N Band, S Mohrlok
Jun 1, 1969·The Journal of Cell Biology·B Bowers, E D Korn
May 1, 1983·The American Journal of Tropical Medicine and Hygiene·C J Austin, L G Warren
Jan 1, 1994·Critical Reviews in Microbiology·S Rodríguez-Zaragoza
Jun 15, 1995·The Biochemical Journal·J Pines
Nov 10, 1998·Experimental Parasitology·M KumagaiT Takeuchi
Feb 27, 2001·Journal of Theoretical Biology·S Cooper
Feb 27, 2001·Molecular and Biochemical Parasitology·A Ganguly, A Lohia
Mar 16, 2001·The Journal of Eukaryotic Microbiology·D LloydA D Russell
Aug 10, 2001·Current Opinion in Microbiology·D Eichinger
Apr 15, 2003·Clinical Microbiology Reviews·Francine Marciano-Cabral, Guy Cabral
Jan 11, 1965·Biochimica Et Biophysica Acta·H S ROSENKRANZ, J A LEVY
Aug 18, 2004·International Journal for Parasitology·Frederick L Schuster, Govinda S Visvesvara
Mar 23, 2007·Nature Reviews. Molecular Cell Biology·Jonathan M W Slack
Feb 22, 2008·International Journal for Parasitology·David S ReinerStaffan G Svärd
Jan 20, 2009·Molecular and Biochemical Parasitology·Glynn R ForsytheTansy C Hammarton
Feb 25, 2009·Nature Reviews. Cancer·Marcos Malumbres, Mariano Barbacid
Sep 9, 2009·Experimental Parasitology·Klára HofstetrováEva Nohýnková
Aug 7, 2010·Advances in Experimental Medicine and Biology·Zbigniew DarzynkiewiczHong Zhao
Aug 24, 2010·Experimental Parasitology·Jarmila KliescikovaEva Nohynkova
May 12, 2012·Molecular and Biochemical Parasitology·Eva BínováEva Nohýnková
Jun 19, 2013·Cell Host & Microbe·Emily S C RittershausChristopher M Sassetti
Aug 27, 2013·Cellular and Molecular Life Sciences : CMLS·Robert J Huber
Jan 18, 2014·BMC Evolutionary Biology·Lihuan CaoLong Yu
May 9, 2014·Frontiers in Cellular and Infection Microbiology·Eduard Torrents
Nov 29, 2014·Nucleic Acids Research·Andrey G BaranovskiyTahir H Tahirov
Oct 3, 2017·Briefings in Bioinformatics·Kazutaka KatohKazunori D Yamada

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.