DNA copy number alterations and expression of relevant genes in mouse thymic lymphomas induced by gamma-irradiation and N-methyl-N-nitrosourea

Cancer Genetics and Cytogenetics
Hyun-Mi KangYeun-Jun Chung

Abstract

The genetic mechanism for the development and progression of a lymphoma is unclear. This study investigated the alterations in the DNA copy number and the expression profiles of the genes located in the altered regions in mouse thymic lymphomas that were induced by two mutagens, gamma-irradiation and N-methyl-N-nitrosourea (MNU). Microarray-based comparative genomic hybridization was used to precisely delineate the boundaries of the altered region. The copy number gains of chromosomes 4 and 5 were observed only in the radiation-induced lymphomas, and gains of chromosomes 10 and 14 were observed only in the MNU-induced lymphomas. Regional copy number losses in chromosomes 11, 16, and 19 appeared frequently in the radiation-induced lymphomas. The cancer-related genes Pten, Ikaros/Znfn1a1, Ercc4, and Top3b were located in the minimal deletion regions. In particular, the expression levels of the Pten, Top3b, and Ikaros genes were downregulated in both lymphoma groups, but the expression level of Ercc4 was downregulated only in the MNU group. This study also examined the expression levels of Sparc, Cxcl1, and Myc (synonym: c-Myc), which are located in the copy number gained chromosomes. Sparc was upregulated specifically in the radi...Continue Reading

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Citations

Apr 12, 2011·Blood·María J BuenoMarcos Malumbres
Jul 19, 2011·World Journal of Biological Chemistry·Philippe Kastner, Susan Chan
Feb 28, 2008·Radiation Research·Robert R KimmelJeffrey L Schwartz
May 28, 2019·Molecular Cytogenetics·Tigran HarutyunyanRouben Aroutiounian
Sep 27, 2019·International Journal of Molecular Sciences·Galina HovhannisyanThomas Liehr

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