DNA damage and Repair Modify DNA methylation and Chromatin Domain of the Targeted Locus: Mechanism of allele methylation polymorphism

Scientific Reports
Giusi RussoEnrico V Avvedimento


We characterize the changes in chromatin structure, DNA methylation and transcription during and after homologous DNA repair (HR). We find that HR modifies the DNA methylation pattern of the repaired segment. HR also alters local histone H3 methylation as well chromatin structure by inducing DNA-chromatin loops connecting the 5' and 3' ends of the repaired gene. During a two-week period after repair, transcription-associated demethylation promoted by Base Excision Repair enzymes further modifies methylation of the repaired DNA. Subsequently, the repaired genes display stable but diverse methylation profiles. These profiles govern the levels of expression in each clone. Our data argue that DNA methylation and chromatin remodelling induced by HR may be a source of permanent variation of gene expression in somatic cells.


Nov 25, 1993·Nature·E LiR Jaenisch
Jun 1, 1996·Trends in Genetics : TIG·M Jasin
Oct 29, 1999·Genes & Development·A J PierceM Jasin
Feb 16, 2002·Science·Job DekkerNancy Kleckner
Feb 16, 2006·Molecular and Cellular Biology·Kishor K BhakatSankar Mitra
Feb 24, 2006·Nature Reviews. Cancer·Stephen B Baylin, Joyce E Ohm
Jul 10, 2007·PLoS Genetics·Concetta CuozzoEnrico V Avvedimento
Feb 2, 2010·Cell Research·Thomas Vaissière, Zdenko Herceg
Feb 18, 2010·Cell Cycle·Yingli SunBrendan D Price
Aug 5, 2010·Nature Reviews. Molecular Cell Biology·Susan C Wu, Yi Zhang
Apr 15, 2011·Nature·Kristine WilliamsKristian Helin
Dec 3, 2011·Nucleic Acids Research·Bongyong LeeMark T Muller
Apr 27, 2013·Epigenetics & Chromatin·Iksoo HuhSoojin V Yi
Oct 22, 2013·Genome Biology·Steve Horvath
Oct 25, 2013·Nature·Rahul M Kohli, Yi Zhang
Jun 21, 2014·Nucleic Acids Research·Udo MüllerHeinrich Leonhardt
Oct 4, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Viktor M PastukhMark N Gillespie


Apr 12, 2017·Scientific Data·Antonio PezoneEnrico V Avvedimento
Jun 29, 2017·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Iolanda AquilaDaniele Torella
Nov 5, 2019·Nucleic Acids Research·Christopher P CaliEdward B Lee
Mar 19, 2020·Biomolecules·Alfonso TramontanoAntonio Pezone
Oct 16, 2019·Genome Biology·Yiqun ZhangChad J Creighton
Feb 10, 2020·Molecular Biology Reports·A A AnuchinaS A Smirnikhina
Aug 21, 2019·Frontiers in Physiology·Noémie GensousPaolo Garagnani
Apr 18, 2019·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Katharina V SchulzeNeil A Hanchard
Feb 21, 2018·Frontiers in Oncology·Marc-Olivier TurgeonGeorge Poulogiannis
Jun 19, 2019·Epigenetics : Official Journal of the DNA Methylation Society·Laura P SuttonKate H Brettingham-Moore
Aug 25, 2019·International Journal of Molecular Sciences·Jin-Hong Kim
May 19, 2019·DNA Repair·Erica Silva, Trey Ideker

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Genotoxic Stress
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