PMID: 7520998Sep 1, 1994Paper

DNA damage and stress protein synthesis induced by oxidative stress proceed independently in the human premonocytic line U937

Mutation Research
M P MinisiniB S Polla

Abstract

Heat shock proteins (HSPs) function as stress-inducible molecular chaperones and exert protective effects against cellular injury. The induction of the HSPs is considered to be mediated by the presence of abnormal proteins within the cell and/or by classical second messengers. Several lines of evidence have however suggested a relationship between DNA damage, HSP induction and thermotolerance. We investigated whether DNA alterations could represent a common signal for the induction of stress protein synthesis during heat shock or exposure to reactive oxygen species in the human premonocytic line U937. We measured, in parallel, DNA damage (both strand breaks and fragmentation) and HSP synthesis (by biometabolic labeling and Western blotting) after exposure to heat shock, hydrogen peroxide, bleomycin, cadmium or erythrophagocytosis. Heat shock induced DNA alterations along with HSP synthesis. In contrast, exposure to hydrogen peroxide or bleomycin induced DNA damage, but no HSP synthesis, suggesting that oxidation-induced DNA damage and HSP synthesis proceed independently in U937 cells. Erythrophagocytosis and cadmium induced the classical HSPs but no detectable DNA damage. Since these latter stresses also induced the oxidation-s...Continue Reading

References

Jun 1, 1992·Journal of Cellular Physiology·D D Mosser, L H Martin
Oct 1, 1992·Experimental Cell Research·K Ohtsuka, A Laszlo
Oct 15, 1991·Biochemical and Biophysical Research Communications·S Kantengwa, B S Polla
Jan 8, 1991·Biochemistry·T J McBrideL A Loeb
Mar 1, 1991·Mutation Research·L F Povirk, M J Austin
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M Clerget, B S Polla
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D D MosserR I Morimoto
Apr 1, 1990·Biochemical Society Transactions·S V LennonT G Cotter
Jan 1, 1986·Annual Review of Biochemistry·S Lindquist
Nov 1, 1989·The American Journal of Physiology·P L Moseley
Aug 1, 1988·International Journal of Radiation Biology·R L AndersonG M Hahn
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S M Keyse, R M Tyrrell
Jul 1, 1986·Experimental Cell Research·K W Lanks
Sep 1, 1985·Journal of Cellular Physiology·R L WartersJ Axtell-Bartlett

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Citations

Nov 30, 1994·Experientia·M R Jacquier-SarlinB S Polla
Feb 4, 2010·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Jung-Woo BaeSeok-Yong Lee
Jan 1, 1997·Free Radical Biology & Medicine·P BrenneisenK Scharffetter-Kochanek
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·B S PollaA Cossarizza
May 16, 2006·The International Journal of Biochemistry & Cell Biology·Jérôme BusserollesMaría José Alcaraz
Oct 4, 2005·Arthritis and Rheumatism·Isabel DevesaMaría José Alcaraz
Aug 31, 2019·Oxidative Medicine and Cellular Longevity·Serena GalatiAnnamaria Buschini
Nov 13, 2002·The Journal of Biological Chemistry·Chuangui Wang, Jiandong Chen

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