DNA damage in blood cells exposed to low-level lasers

Lasers in Surgery and Medicine
Luiz Philippe da Silva SergioAdenilson de Souza da Fonseca

Abstract

In regenerative medicine, there are increasing applications of low-level lasers in therapeutic protocols for treatment of diseases in soft and in bone tissues. However, there are doubts about effects on DNA, and an adequate dosimetry could improve the safety of clinical applications of these lasers. This work aimed to evaluate DNA damage in peripheral blood cells of Wistar rats induced by low-level red and infrared lasers at different fluences, powers, and emission modes according to therapeutic protocols. Peripheral blood samples were exposed to lasers and DNA damage was accessed by comet assay. In other experiments, DNA damage was accessed in blood cells by modified comet assay using formamidopyrimidine DNA glycosylase (Fpg) and endonuclease III enzymes. Data show that exposure to low-level red and infrared lasers induce DNA damage depending on fluence, power and emission mode, which are targeted by Fpg and endonuclease III. Oxidative DNA damage should be considered for therapeutic efficacy and patient safety in clinical applications based on low-level red and infrared lasers.

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Citations

Sep 27, 2018·Photomedicine and Laser Surgery·Magda Patrícia FurlanettoRodrigo Della Méa Plentz
Jan 8, 2019·International Journal of Radiation Biology·Ruwaidah A MussttafAwadhesh N Jha
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Apr 27, 2018·Lasers in Medical Science·Larissa Alexsandra da Silva Neto TrajanoAdenilson de Souza da Fonseca
Jun 2, 2017·Redox Biology·Alfonso Blázquez-Castro
May 20, 2021·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Larissa Alexsandra da Silva Neto TrajanoAdenilson de Souza da Fonseca

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