DNA damage in breast epithelial cells: detection by the single-cell gel (comet) assay and induction by human mammary lipid extracts

Carcinogenesis
F L MartinDavid H Phillips

Abstract

The presence of DNA damage in primary cultures of human mammary epithelial cells (HMECs), and the ability of extracts of human mammary lipid to cause such damage, has been investigated. Lipid extracts, prepared by a solid-phase procedure, and HMECs were obtained from breast tissue removed from healthy women (ages 18-50 years) who were resident in the UK and undergoing elective reduction mammoplasties. DNA single strand breaks (SSBs) were detected using the single-cell gel assay (comet assay) with alkaline electrophoresis (pH 12.3) and quantified by measuring comet tail length (CTL) (microm). Untreated HMECs and HMECs incubated (30 min, 37 degrees C) with a mammary lipid extract, with or without DNA-repair inhibitors hydroxyurea (HU) and cytosine arabinoside (ara-C), were examined. Ionizing radiation was used as a positive control. An active lipid extract gave a linear dose-response over the range 2.0-12.2 g equivalents. When MCL-5 cells, a line of metabolically-competent human lymphoblastoid cells, were used to compare the DNA-damaging properties of lipid extracts from six different donors, significant interindividual variations (median CTLs were 15.0, 53.5, 32.5, <4.0, <4.0 and 77.5 microm respectively) were observed. In eight...Continue Reading

Citations

Mar 10, 2001·International Journal of Cancer. Journal International Du Cancer·N Rajaee-BehbahaniH Bartsch
Oct 12, 2000·Mutation Research·F L MartinD H Phillips
Aug 25, 2001·The British Journal of Nutrition·F BrancaH Verhagen
Oct 16, 2013·Proceedings of the National Academy of Sciences of the United States of America·Saijun FanEliot M Rosen
Mar 29, 2002·Environmental and Molecular Mutagenesis·David H PhillipsPhilip L Grover
Mar 29, 2002·Environmental and Molecular Mutagenesis·Donghui LiJohn DiGiovanni
Mar 29, 2002·Environmental and Molecular Mutagenesis·Kathleen A Hill, Steve S Sommer
Feb 16, 2016·Cancer Cell International·Marco Allán Pérez-SolisArturo Aguilar-Rojas
Sep 18, 2004·Cancer Letters·Narasimhan RagavanFrancis L Martin
Jun 11, 2002·Biochemical and Biophysical Research Communications·Francis L MartinPhilip L Grover
Apr 13, 1999·Biochemical and Biophysical Research Communications·F L MartinD H Phillips
Oct 29, 1998·Biochemical and Biophysical Research Communications·F L MartinP L Grover
Jun 22, 2000·Environmental and Molecular Mutagenesis·G NeurathH Marquardt
Dec 24, 2002·Prostate Cancer and Prostatic Diseases·F L MartinD H Phillips
Mar 16, 2001·Breast Cancer Research : BCR·R Scully
Nov 26, 1999·Cancer Detection and Prevention·D LiW N Hittelman
Mar 6, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·E RojasM Valverde

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