DNA damage in peripheral blood lymphocytes in patients during combined chemotherapy for breast cancer

Mutation Research
Patricia Sánchez-SuárezLuis Benítez-Bribiesca

Abstract

Combined chemotherapy is used for the treatment of a number of malignancies such as breast cancer. The target of these antineoplastic agents is nuclear DNA, although it is not restricted to malignant cells. The aim of the present study was to assess DNA damage in peripheral blood lymphocytes (PBLs) of breast cancer patients subjected to combined adjuvant chemotherapy (5-fluorouracil, epirubicin and cyclophosphamide, FEC), using a modified comet assay to detect DNA single-strand breaks (SSB) and double-strand breaks (DSB). Forty-one female patients with advanced breast cancer before and after chemotherapy and 60 healthy females participated in the study. Alkaline and neutral comet assays were performed in PBLs according to a standard protocol, and DNA tail moment was measured by a computer-based image analysis system. Breast cancer patients before treatment had higher increased background levels of SSB and DSB as compared to healthy women. During treatment, a significant increase in DNA damage was observed after the 2nd cycle, which persisted until the end of treatment. Eighty days after the end of treatment the percentage of PBLs with SSB and DSB remained elevated, but the magnitude of DNA damage (tail moment) returned to basel...Continue Reading

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Citations

Feb 14, 2009·Biological Trace Element Research·Alessandra de Santana Braga Barbosa RibeiroElisângela de Paula Silveira-Lacerda
Oct 26, 2011·Wiener klinische Wochenschrift·Petra FikrováZdeněk Zadák
Jan 20, 2010·Mutation Research·Cristiane Cademartori DanesiMauricio Lehmann
Dec 8, 2009·Mutation Research·Silvia SterponeVittorio Donato
Dec 23, 2008·Mutation Research·Shinji OikawaShosuke Kawanishi
Nov 18, 2015·Oxidative Medicine and Cellular Longevity·Antonio Luiz Gomes JúniorAna Amélia de Carvalho Melo Cavalcante
May 24, 2017·Journal of Translational Medicine·Ning RenChen-Hao Zhou
Nov 16, 2018·Physiological Research·M KuchařováA Patková
Jun 6, 2013·Experimental and Therapeutic Medicine·Yan ZhangShuchai Zhu
Feb 28, 2015·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Delphine DenoyerRodney J Hicks

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