DNA damage, repair, and replication in selenite-induced cataract in rat lens

Current Eye Research
L L HuangG E Bunce

Abstract

DNA synthesis was evaluated in vitro by measuring incorporation of 3H-thymidine in rat lens following systemic delivery of a cataractogenic dose of selenite. Among early metabolic changes observed in the lenses of rats receiving a single dose of 30 nmol Na2SeO3/g body weight was a 30% decrease in DNA replication in lens epithelium occurring between 6 and 12 h after administration of the selenite. This change was followed by an 80% increase in replication by 24 h. Thymidine incorporation in DNA remained elevated compared to controls through 96 h. Unscheduled DNA synthesis was found to be approximately 10% of the total DNA formed, but there was a 30% and 70% increase of this putative DNA repair in the lenses from selenite-treated animals at 6 and 24 h after the injection. Using the alkaline unwinding assay, the proportion of single-strand DNA in lenses from selenite-treated animals increased after 24 h. This estimate of DNA damage was greater in lenses after 96 h. Each component of DNA metabolism: damage, repair, and replication, was affected by the occurrence of selenite stress in lens. These changes both preceded and accompanied nuclear cataract formation.

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Citations

Jun 29, 2011·Journal of Toxicology and Environmental Health. Part a·Vanessa ValdiglesiasBlanca Laffon
Apr 1, 1992·Current Eye Research·T R ShearerM Azuma
Nov 26, 2015·Ophthalmology and Therapy·Samuel KyeiEmmanuel Akomanin Asiamah
Jul 6, 2014·Biochimica Et Biophysica Acta·Periyasamy PalsamyToshimichi Shinohara
Apr 13, 2002·Experimental Eye Research·Takeshi NakajimaThomas R Shearer
Dec 25, 2009·Archives of Toxicology·Vanessa ValdiglesiasBlanca Laffon
Jun 23, 2019·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Farzin KamariHassan Aslani
Aug 31, 2000·Biochemical and Biophysical Research Communications·Y TamadaT R Shearer

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