PMID: 11319142Apr 25, 2001Paper

DNA damaging agents increase gadd153 (CHOP-10) messenger RNA levels in bovine preimplantation embryos cultured in vitro

Biology of Reproduction
N Fontanier-RazzaqW D Rees

Abstract

DNA damage and other forms of stress are believed to be important factors in reducing the efficiency of in vitro embryo transfer techniques in farm animals. The expression of mRNAs from stress-responsive genes such as gadd153 (CHOP-10, ddit3) may provide a means of assessing the quality of embryos produced in vitro. Treatment of bovine granulosa cell cultures with the DNA-damaging agents, methyl methane-sulphonate (MMS) or sodium arsenite, induced the expression of an mRNA, which hybridized with the hamster gadd153 cDNA. Part of the corresponding bovine cDNA was amplified by nested polymerase chain reaction (PCR), cloned, and sequenced. Using a sensitive reverse transcriptase-PCR assay we have investigated the expression of gadd153 and beta-actin in blastocyst-stage bovine embryos treated with MMS or sodium arsenite. Both agents produced an increase in the ratio of gadd153 mRNA relative to beta-actin. These results show that there are changes in gene expression in blastocyst-stage bovine embryos in response to genotoxic stress, suggesting that an increase in gadd153 mRNA is a useful marker of DNA damage and metabolic stress in preimplantation embryos.

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Citations

Apr 29, 1998·Trends in Neurosciences·R NobiliJ Ashmore
Oct 23, 2002·Biology of Reproduction·Claude RobertMarc-André Sirard
Jun 25, 2004·Biology of Reproduction·Tom P FlemingJudith J Eckert
Sep 27, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Christopher A Maloney, William D Rees
Dec 16, 2006·Journal of Cellular Biochemistry·David W Scott, George Loo
May 30, 2015·Cell and Tissue Research·Yanzhou YangCheng Zhang
Mar 31, 2015·Toxicology and Applied Pharmacology·William M WardGeorge Loo
Aug 7, 2002·The Journal of Nutrition·Nathalie Fontanier-RazzaqWilliam D Rees
Mar 5, 2005·Reproduction, Fertility, and Development·Christine WrenzyckiHeiner Niemann

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