PMID: 40124Sep 1, 1979

DNA-damaging and mutagenic effects of 1,2-dimethylhydrazine on Bacillus subtilis repair-deficient mutants

Mutation Research
I C FelknerB C Wells

Abstract

Mutagenic, DNA-damaging, and in vivo alteration of DNA have been demonstrated for 1,2-dimethylhydrazine (DMH), a potent inducer of adenocarcinomas of the large intestine and colon of rats. These activities are pH-dependent, with 6.5 giving optimum response. There was no requirement for metabolic activation with rat-liver S9 mix when the appropriate Bacillus subtilis mutant strains were used. The Rec- strains recA8 and mc-1 were greater than 300-fold more sensitive to the DNA-damaging activity of DMH than was their isogenic wild-type parent. The DNA isolated from DMH-treated mc-1 had altered spectroscopic characteristics, and gave a greatly reduced transformation efficiency. Treatment of B. subtilis strain TKJ6321 with DMH at pH 6.5 induced His+, Met+ mutations in substantial numbers at low concentrations of this chemical. The use of B. subtilis mutants in these studies has therefore made it possible to demonstrate mutagenic and DNA-damaging activity in bacteria for this potent carcinogenic chemical.

References

Mar 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J McCann, B N Ames
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J McCannB N Ames
Feb 1, 1976·Proceedings of the Society for Experimental Biology and Medicine·B S ReddyE L Wynder
Aug 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·B N AmesF D Lee
Jan 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·E EPHRATI-ELIZURS ZAMENHOF
Oct 15, 1958·Proceedings of the National Academy of Sciences of the United States of America·J Spizizen

Citations

Apr 1, 1982·Bulletin of Environmental Contamination and Toxicology·K W Brown, K C Donnelly
Aug 1, 1992·Mutation Research·V S Houk
Dec 1, 1982·Mutation Research·E G RoganB Toth

Related Concepts

Natto Bacteria
Dimethylhydrazines
Base Excision Repair
DNA, Bacterial
Hydrogen-Ion Concentration
Methylhydrazines
Clastogens
RNA Conformation
Spectrophotometry

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