DNA degradation in liquid-based cytology and its comparison with conventional smear

Diagnostic Cytopathology
Wook Youn KimTae Sook Hwang

Abstract

In fine needle aspiration biopsy (FNAB) of thyroid nodules, LBC is adopted in most of the hospitals and clinics in Korea for its convenience. BRAF mutation test has been introduced as an important ancillary test, but its applicability has not been completely proven with LBC samples. Five aspirates from thyroidectomy specimens were simultaneously processed into LBC and CS slides, in which BRAF mutation tests were performed using three primer sets with PCR products of 72, 164, and 226 base pairs (bp) at 6, 9, and 12 months after processing. In addition, BRAF mutation tests were performed in nine clinical samples that had been prepared by LBC or CS and stored for 3-5 years after processing. At 9 months after processing, LBC failed to provide DNA of sufficient quality for PCR, whereas CS succeeded with primers for amplifying a 226 bp fragment. Furthermore, CS had successful amplification of DNA despite a delay of more than 1 year. The failure of DNA amplification in LBC was overcome by using primers to amplify shorter PCR products, suggesting that DNA degradation occurred in LBC. However, false positive or negative results were observed in primers for amplifying shorter size. The kind of preservative solutions used in LBC did not a...Continue Reading

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Citations

Aug 25, 2016·Archives of Pathology & Laboratory Medicine·Anjali Saqi
Mar 17, 2018·Archives of Pathology & Laboratory Medicine·Deepali Jain, Sinchita Roy-Chowdhuri
Jul 11, 2018·Cytopathology : Official Journal of the British Society for Clinical Cytology·Helena BarrocaPio Zeppa
Aug 29, 2018·Cytopathology : Official Journal of the British Society for Clinical Cytology·Aruna Nambirajan, Deepali Jain
Apr 16, 2016·Archives of Pathology & Laboratory Medicine·Sinchita Roy-ChowdhuriYasushi Yatabe
Sep 2, 2020·Virchows Archiv : an International Journal of Pathology·Yukiko MatsuoYukitoshi Satoh
Oct 31, 2021·Cytopathology : Official Journal of the British Society for Clinical Cytology·Anthony Maddox, Louise M Smart

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