DNA degradation with ozone

International Journal of Biological Macromolecules
Franco Cataldo

Abstract

DNA was ozonized in solution and the reaction was followed with polarimetry and with iodimetry. Polarimetry was used to determine the molar ratio DNA/O(3) when the DNA optical activity vanishes completely. At a molar ratio DNA/O(3)=2.3 the supramolecular structure of DNA collapses completely. Instead, iodimetry shows that the ozonolysis proceeds until all the nucleobases have been destroyed, an event which occurs at a molar ratio DNA/O(3)=1.1. The ozonolysis of DNA was also followed spectrophotometrically. DNA is reactive with ozone also in the solid state, as fixed bed. Clear indication about its oxidation derives from the FT-IR spectra from polarimetric measurements and from thermal analysis performed by thermogravimetric analysis (TGA), differential thermogravimetric analysis (DTG) and from differential thermal analysis (DTA). Particular remarkable is the fact that RNA has been found much less reactive toward ozone in the solid state than DNA.

References

Aug 11, 1975·Differentiation; Research in Biological Diversity·D Bellamy, S M Hinsull

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Citations

Apr 17, 2013·Canadian Journal of Microbiology·Noora H J RäsänenEila Torvinen
Sep 27, 2012·BMC Research Notes·Tung Nguyen-HieuMichel Drancourt
Apr 6, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Suk-Nam KangOk-Hwan Lee
Jan 21, 2015·Inhalation Toxicology·Kevin C BaldridgeLydia M Contreras
Feb 13, 2014·International Journal of Radiation Biology·Maria RaposoRobert W McCullough
Apr 20, 2017·Journal of Prosthodontics : Official Journal of the American College of Prosthodontists·Ahila S Chidambaranathan, Muthukumar Balasubramanium
Sep 7, 2020·The Science of the Total Environment·Mutong NiuFangxia Shen
Mar 30, 2017·The Journal of Contemporary Dental Practice·Rafaella M ChavesLawrence G Lopes
Jan 9, 2021·Chemical Research in Toxicology·J Richard WagnerJean Cadet

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