DNA-dependent ATPases from Escherichia coli K12

Cold Spring Harbor Symposia on Quantitative Biology
E RichetM Kohiyama


Four DNA-dependent ATPases have been isolated from E. coli extracts. ATPases I and III, both sensitive to NEM, require denatured DNA but differ in their heat sensitivity, elution from DEAE-cellulose, and sedimentation coefficient. ATPases II and IV are both resistant to NEM. ATPase II requires partially denatured DNA, whereas ATPase IV can be stimulated by SS DNA. ATPase I is a DNA-unwinding enzyme; ATPase II may be involved in recombination.


Mar 3, 2010·Annual Review of Biophysics·Wei Yang

Related Concepts

DNA-dependent ATPase
Ion-Exchange Chromatography Procedure
DNA Replication
ATP-Dependent DNA Helicases
DNA, Single-Stranded
Alkalescens-Dispar Group
Recombination, Genetic
Substrate Specificity

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