DNA-dependent DNA polymerase species in male germ cells of the mouse

Cell Differentiation
P GrippoV Monesi

Abstract

Quasi-homogeneous fractions of male mouse germ cells at definite stages of meiosis and spermiogenesis were obtained by using a separation method based on sedimentation velocity in an albumin gradient. In the various cell types, the total DNA-dependent DNA polymerase activity was determined, and the major enzymatic forms were characterized. The DNA polymerase species present in premeiotic, meiotic and post-meiotic cells were analyzed by glycerol gradient sedimentation. Two types of DNA polymerase were identified in fractions enriched in spermatogonia and preleptotene spermatocytes. One showed a sedimentation coefficient of about 7.5 S and was sensitive to N-ethylmaleimide (NEM); the other exhibited a sedimentation coefficient between 3 and 4 S and was resistant to NEM. On the basis of their sedimentation coefficients, their sensitivity to NEM and their template specificities, these 2 enzymes were identified respectively as alpha and beta DNA polymerases as reported in mammals. The gradient analysis performed on fractions enriched in meiotic and post-meiotic cells revealed the presence of DNA polymerase beta only. A quantitative analysis showed that the activity of the DNA polymerase beta reaches a maximum at middle-late pachyten...Continue Reading

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Citations

Dec 1, 1978·The Anatomical Record·H E ChemesM Dym
Nov 1, 1983·Mechanisms of Ageing and Development·F HanaokaM Yamada
Jul 1, 1989·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·P OrlandoP Grippo
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Jan 1, 1989·Archives of Andrology·M FujisawaS Kamidono
Aug 1, 1984·Experimental Cell Research·P OrlandoR Geremia

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