DNA diagnosis by capillary electrophoresis and microfabricated electrophoretic devices

Expert Review of Molecular Diagnostics
Jianhua QinBingcheng Lin

Abstract

DNA diagnosis is experiencing an impressive progression towards the development of novel technology to identify various clinically relevant categories of genetic changes and to meet the exponential growth of genomics. The introduction of capillary electrophoresis has dramatically accelerated the completion of the first draft of the human DNA sequence in the Human Genome Project, and thus, has become the method of choice for analysis of various genetic variants. The recent development of microfabricated electrophoretic devices has led to the possibility of integrating multiple sample handling with the actual measurement steps required for automation of molecular diagnostics. This review highlights the most recent progress in capillary electrophoresis and electrophoretic microdevices for DNA-based diagnostics, including the important areas of genotyping for point mutation, single nucleotide polymorphisms, short tandem repeats and organism identification. The application of these techniques for infectious and genetic disease diagnosis, as well as forensic identification purpose, are covered. The promising development and the challenges for techinical problems are also discussed.

References

May 26, 1998·Analytical Chemistry·M S IbrahimP Belgrader
Oct 17, 1998·Science·M A BurnsD T Burke
Jan 14, 2000·Electrophoresis·J C McDonaldG M Whitesides
Jan 5, 2001·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·S A WangA Saah
Mar 14, 2002·Electrophoresis·Elisabeth Verpoorte
Mar 21, 2002·Clinical Genetics·C D O'ConnellK l Richie
Jul 11, 2002·Human Genetics·Zhaoxia RenJ Fielding Hejtmancik
Aug 2, 2002·Journal of Chromatography. a·Wenwan Zhong, Edward S Yeung
Sep 28, 2002·Science·Todd ThorsenStephen R Quake
Dec 20, 2002·Bioinformatics·A Riva, I S Kohane

❮ Previous
Next ❯

Citations

May 19, 2006·Expert Review of Molecular Diagnostics·Luc Bissonnette, Michel G Bergeron
Jul 17, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Abdelfattah M AttallahGamal E Shiha
Mar 19, 2014·Forensic Science International. Genetics·Huaigu ZhouWeiguo Zheng
Oct 28, 2006·Biotechnology Journal·Bingcheng LinJianhua Qin
Jan 20, 2006·Electrophoresis·Franco Tagliaro, Federica Bortolotti
Nov 22, 2005·Clinical Chemistry·Sam F Y Li, Larry J Kricka

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Nucleic Acids Research
Krzysztof Sobczak, Wlodzimierz J Krzyzosiak
Molecular Diagnosis : a Journal Devoted to the Understanding of Human Disease Through the Clinical Application of Molecular Biology
Bulbin Sunar-ReederC D O'Connell
Journal of Chromatography. B, Biomedical Applications
Z Nackerdien, D Atha
Chemistry & Biology
Lara K Mahal
© 2022 Meta ULC. All rights reserved