DNA directed damage using a brominated DAPI derivative

Chemical Communications : Chem Comm
Elyse M DigbyAndrew A Beharry

Abstract

Photodynamic therapy (PDT) is a clinically approved cancer treatment that uses light, oxygen and a photosensitizer to produce localized reactive oxygen species (ROS). Due to the short lifetime of ROS, the location of the photosensitizer in the cell is believed to be the key determinant governing the outcome of PDT. To explore the effect of direct association between a photosensitizer and DNA a well know DNA-binding dye, DAPI, was converted into a photosensitizer. Br-DAPI - unlike native DAPI - upon irradiation produces ROS. We demonstrate that the ROS are only effective in inducing dsDNA breaks when Br-DAPI is bound to DNA. In cancer cells (A549) Br-DAPI causes rapid light dependent cell death. This work supports the design of photosensitizers which bind with high affinity to the DNA of target cells for potentially more effective PDT.

References

Sep 1, 1995·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·J Kapuscinski
Aug 18, 2000·Immunology and Cell Biology·A A RosenkranzA S Sobolev
May 21, 2003·Chemical Research in Toxicology·Giampietro ViolaArianna Barbafina
Aug 11, 2006·Trends in Molecular Medicine·Wynand P Roos, Bernd Kaina
May 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Gang ZhengBrian C Wilson
Sep 8, 2007·The International Journal of Biochemistry & Cell Biology·Louise CopleyVirna D Leaner
Jun 10, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Ioanna StamatiMahendra P Deonarain
Mar 2, 2011·Nature Reviews. Drug Discovery·Oliver KeppGuido Kroemer
Mar 13, 2012·International Journal of Medical Sciences·Rajamanickam BaskarKheng-Wei Yeoh
Jul 17, 2012·Cytotechnology·Alexander P Demchenko
Jan 1, 2011·Cancers·Pawel MrozMichael R Hamblin
Sep 3, 2013·Photodiagnosis and Photodynamic Therapy·Dan Dan XuWilliam Chi Shing Cho
Nov 6, 2013·Photochemistry and Photobiology·Qinsi ZhengScott C Blanchard
Dec 1, 2004·Photodiagnosis and Photodynamic Therapy·Ana P CastanoMichael R Hamblin
Sep 13, 2014·Chemistry : a European Journal·Cristina MariGilles Gasser
Jun 3, 2015·Accounts of Chemical Research·Natalia Y TretyakovaShaofei Ji
Sep 4, 2015·International Journal of Molecular Sciences·Isabel O L BacellarMauricio S Baptista
Jan 8, 2016·Redox Biology·Celia QuijanoAndrés Trostchansky
Feb 11, 2016·The Biochemical Journal·Heidi Abrahamse, Michael R Hamblin
Jan 14, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rui ShiJinfeng Wei
Oct 28, 2017·Cell Research·Yingying ZhangJiahuai Han
Nov 18, 2017·Archiv der Pharmazie·Kamilia M AminFardous F Elsenduny
Nov 23, 2017·Photochemistry and Photobiology·Masahiko Taniguchi, Jonathan S Lindsey
Jan 10, 2018·Photochemistry and Photobiology·Jeffrey D LaskinNed D Heindel
Sep 28, 2018·Advanced Materials·Wei-Hai ChenXian-Zheng Zhang
Dec 16, 2018·Angewandte Chemie·Pingyu ZhangPeter J Sadler

❮ Previous
Next ❯

Citations

Sep 9, 2020·Chembiochem : a European Journal of Chemical Biology·Rahul RanaMark Nitz
Sep 11, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Qun GuanYu-Bin Dong
Oct 19, 2021·Chembiochem : a European Journal of Chemical Biology·Congcong ZhangLin Li

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Postȩpy higieny i medycyny doświadczalnej
Marcin KubiakRafał Nowak
Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Barbara W HendersonHeinz Baumann
Anais Da Academia Brasileira De Ciências
Mariana Q MesquitaMaria Amparo F Faustino
© 2021 Meta ULC. All rights reserved