DNA-directed protein immobilization on mixed self-assembled monolayers via a streptavidin bridge

Langmuir : the ACS Journal of Surfaces and Colloids
Jon LaddShaoyi Jiang

Abstract

The simultaneous detection of multiple analytes is an important consideration for the advancement of biosensor technology. Currently, few sensor systems possess the capability to accurately and precisely detect multiple antigens. This work presents a simple approach for the functionalization of sensor surfaces suitable for multichannel detection. This approach utilizes self-assembled monolayer (SAM) chemistry to create a nonfouling, functional sensor platform based on biotinylated single-stranded DNA immobilized via a streptavidin bridge to a mixed SAM of biotinylated alkanethiol and oligo(ethylene glycol). Nonspecific binding is minimized with the nonfouling background of the sensor surface. A usable protein chip is generated by applying protein-DNA conjugates which are directed to specific sites on the sensor chip surface by utilizing the specificity of DNA hybridization. The described platform is demonstrated in a custom-built surface plasmon resonance biosensor. The detection capabilities of a sensor using this protein array have been characterized using human chorionic gonadotropin (hCG). The platform shows a higher sensitivity in detection of hCG than that observed using biotinylated antibodies. Results also show excellen...Continue Reading

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Citations

May 3, 2011·Journal of the American Chemical Society·Matteo PalmaShalom J Wind
Sep 20, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Ognen Pop-GeorgievskiPatrick Koelsch
Dec 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Aric OpdahlLloyd J Whitman
Jun 6, 2008·Organic & Biomolecular Chemistry·Mike KleinertThisbe K Lindhorst
Jan 26, 2011·Chemical Society Reviews·Debasis Samanta, Amitabha Sarkar
Nov 28, 2014·Sensors·Satish Balasaheb NimseTaisun Kim
Jan 7, 2011·Trends in Biotechnology·Chung-Huei K Wang, Suzie H Pun
Jul 28, 2009·Biomaterials·Shengfu ChenShaoyi Jiang
May 2, 2009·Journal of Colloid and Interface Science·Hyunsook KimHaiwon Lee
Sep 30, 2008·Biomaterials·Guozhu LiShaoyi Jiang
Nov 23, 2006·Journal of Immunological Methods·Nathalie SchollerNicole Urban
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Apr 3, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Surachet DuanghathaipornsukDong-Shik Kim
Feb 18, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Michael SeifertHans-Joachim Galla
Dec 2, 2009·Biomacromolecules·Peter J RothPatrick Theato
Mar 14, 2008·The Journal of Physical Chemistry. B·Bat Ami GotlivRon Naaman
May 15, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Nirmalya BallavMichael Zharnikov
Feb 21, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Amir SyahirHisakazu Mihara
Feb 20, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Andrew J GrossAlison J Downard
Feb 12, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Beibei ZhangGuosheng Cheng

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