DNA-DNA homology among lactose- and sucrose-fermenting transconjugants from Lactococcus lactis strains exhibiting reduced bacteriophage sensitivity.

Applied and Environmental Microbiology
J L SteeleL L McKay

Abstract

DNA-DNA homology between a reduced bacteriophage sensitivity (Rbs+) probe and DNA from both Rbs+ and Rbs- Lactococcus lactis strains was examined. Homology was detected between the probe and five plasmids (pCI750, pCC34, pEB56, pNP2, and pJS88) isolated from lactose-positive Rbs+ transconjugants and between the probe and genomic DNA of a sucrose-positive Rbs+ transconjugant. Additionally, hybridizations conducted between the probe and plasmids reported to encode abortive bacteriophage infection indicated homology with pTR2030 but not with pBF61 and pGBK17. The results suggest that a common genetic determinant(s) may be present in a variety of lactococcal plasmids coding for Rbs+.

References

Feb 1, 1988·Journal of Dairy Science·B R FrosethL L McKay
Mar 1, 1988·Biochimie·M E Sanders
Aug 1, 1984·Applied and Environmental Microbiology·J K Kondo, L L McKay
Sep 1, 1983·Applied and Environmental Microbiology·D G Anderson, L L McKay
Jul 1, 1980·Applied and Environmental Microbiology·L L McKayP M Walsh
Mar 1, 1985·Applied and Environmental Microbiology·C F Gonzalez, B S Kunka
Jun 1, 1975·Applied Microbiology·B E Terzaghi, W E Sandine

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Citations

Feb 11, 2015·Annual Review of Food Science and Technology·Larry McKay

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