DNA-DNA kissing complexes as a new tool for the assembly of DNA nanostructures

Nucleic Acids Research
Anna BarthManfred Focke

Abstract

Kissing-loop annealing of nucleic acids occurs in nature in several viruses and in prokaryotic replication, among other circumstances. Nucleobases of two nucleic acid strands (loops) interact with each other, although the two strands cannot wrap around each other completely because of the adjacent double-stranded regions (stems). In this study, we exploited DNA kissing-loop interaction for nanotechnological application. We functionalized the vertices of DNA tetrahedrons with DNA stem-loop sequences. The complementary loop sequence design allowed the hybridization of different tetrahedrons via kissing-loop interaction, which might be further exploited for nanotechnology applications like cargo transport and logical elements. Importantly, we were able to manipulate the stability of those kissing-loop complexes based on the choice and concentration of cations, the temperature and the number of complementary loops per tetrahedron either at the same or at different vertices. Moreover, variations in loop sequences allowed the characterization of necessary sequences within the loop as well as additional stability control of the kissing complexes. Therefore, the properties of the presented nanostructures make them an important tool for...Continue Reading

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Citations

Sep 3, 2016·Journal of the American Chemical Society·Pei Guo, Sik Lok Lam
Aug 27, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Claudia AddamianoJean-Marc Escudier
Dec 23, 2017·Nucleic Acids Research·Laurent AzémaJean-Jacques Toulmé
Jul 15, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Irena WielebaPaweł Krawczyk
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alberto Mills, Federico Gago

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