Oct 1, 1977

DNA double-strand breaks and alkali-labile bonds produced by bleomycin

Nucleic Acids Research
L F PovirkF Hutchinson

Abstract

Both in linear T2 DNA, analyyzed by velocity sedimentation, and in supercoiled Col EL DNA, analyzed by gel electrophoresis, the number of double-strand breaks produced by bleomycin was directly propotional to the number of single-strand breaks and was far greater than the number expected from random coincidence of single-strand breaks, suggesting that the bleomycin-induced double-strand breaks occur as an independent event. In Col EL DNA, at least twice as many single-strand breaks were found under alkaline assay conditions as were found under neutral conditions, showing the production of alkaline-labile bonds by bleomycin.

  • References10
  • Citations140
  • References10
  • Citations140

Citations

Mentioned in this Paper

Centrifugation, Density Gradient
DNA, Viral
Bleomycin
Sedimentation Procedure
Assay
Hydrogen-Ion Concentration
DNA, Bacterial
Gel Electrophoresis (Lab Technique)
DNA, Single-Stranded
DNA, Double-Stranded

About this Paper

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