PMID: 6982882Sep 1, 1982Paper

DNA double strand breaks in Ehrlich ascites tumour cells at low doses of x-rays. I. Determination of induced breaks by centrifugation at reduced speed

International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
D Blöcher

Abstract

DNA double strand breaks (dsb) were determined in Ehrlich ascites tumour cells at doses down to 5 Gy. The method is based on the separation of DNA from other components by heating in a solution of pronase and detergents held in wide-mouth syringes, which were also used to facilitate the application of the released high molecular weight DNA to sucrose gradients. Purified DNA was sedimented in neutral sucrose gradients at low speed to reduce speed artifacts. The sedimentation profiles were analysed using a computer program and the number of dsb was determined by simulation of random breaks in the mass distribution of the control sample and by comparison of this simulated profile with that of the irradiated one. The number of dsb formed was proportional to X-ray dose in the range of 5 to 2000 Gy. The induction per dose was found to be nmr-1 D-1 = (11.7 +/- 2) x 10(-12) Gy-1.

References

Sep 1, 1979·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·M Frankenberg-SchwagerC Adamczyk
Oct 25, 1979·Biochimica Et Biophysica Acta·W Krauth, D Werner
Feb 25, 1978·Journal of Molecular Biology·D PettijohnS van Scoyk
Jan 16, 1976·Molecular & General Genetics : MGG·M A Resnick, P Martin
Jul 1, 1976·Biophysical Chemistry·D W ApplebyJ E Hearst
Dec 13, 1976·Biochimica Et Biophysica Acta·R W Clark, C S Lange
Dec 1, 1976·Biophysical Chemistry·F Hutchinson, F Krasin
Jul 1, 1976·Biophysical Chemistry·B H ZimmC B Zimm
Jan 1, 1967·Biochimica Et Biophysica Acta·A G LevisM Errera
Jul 1, 1965·Journal of Molecular Biology·J Eigner, P Doty
Jan 1, 1973·Chromosoma·R Kavenoff, B H Zimm
Dec 30, 1972·Journal of Molecular Biology·R Kavenoff
Jan 1, 1974·Methods in Enzymology·R J BrittenB R Neufeld
Oct 15, 1970·Biochimica Et Biophysica Acta·A R Lehmann, M G Ormerod
Jul 29, 1980·Biochimica Et Biophysica Acta·D WernerH V Hershey
Sep 1, 1980·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·P E Bryant, D Blöcher
Sep 1, 1982·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·D Blöcher, W Pohlit
Jun 1, 1980·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·G Iliakis
Oct 1, 1961·Journal of Molecular Biology·C LEVINTHAL, P F DAVISON
May 1, 1975·Biophysical Chemistry·E J Ralston, V N Schumaker

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Citations

Dec 1, 1990·Photochemistry and Photobiology·D Schulte-FrohlindeH Görner
Mar 1, 1988·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·D E Charlton, J L Humm
Jul 1, 1985·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·P E Bryant
Oct 1, 1985·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·I R Radford, G S Hodgson
Feb 1, 1994·International Journal of Radiation Biology·M C Mühlmann-Díaz, J S Bedford
May 1, 1994·International Journal of Radiation Biology·M BelliP Tiveron
Mar 1, 1990·International Journal of Radiation Biology·J Y Ostashevsky
Sep 1, 1990·International Journal of Radiation Biology·T J McMillanJ H Peacock
Jun 9, 2012·Mutation Research·Eileen PernotElisabeth Cardis
Jan 1, 1987·Chromosoma·J L Murata-Collins, R W Clark
Oct 20, 2018·Radiation Research
Jan 1, 1987·Radiation and Environmental Biophysics·L K Mee, S J Adelstein
Jan 1, 1990·Radiation and Environmental Biophysics·M Frankenberg-Schwager
Aug 7, 2012·Journal of Mathematical Biology·Yasumasa Saisho, Atsushi Ito
Aug 15, 2014·Physics in Medicine and Biology·John C Sutherland
Aug 26, 1998·International Journal of Radiation Biology·K M PriseB Stenerlöw
Jan 1, 1991·Pharmacology & Therapeutics·M C EliaM O Bradley
Jan 1, 1991·Pharmacology & Therapeutics·K W Kohn
Jan 1, 1991·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·S J WhitakerT J McMillan

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