PMID: 6163590Jan 1, 1981Paper

DNA electron microscopy

CRC Critical Reviews in Biochemistry
C Brack

Abstract

In recent years DNA electron microscopy has become a tool of increasing interest in the fields of molecular genetics and molecular and cell biology. Together with the development of in vitro recombination and DNA cloning, new electron microscope techniques have been developed with the aim of studying the structural and functional organization of genetic material. The most important methods are based on nucleic acid hybridizations: DNA-DNA hybridization (heteroduplex, D-loop), RNA-DNA hybridization (R-loop), or combinations of both (R-hybrid). They allow both qualitative and quantitative analysis of gene organization, position and extension of homology regions, and characterization of transcription. The reproducibility and resolution of these methods make it possible to map a specific DNA region within 50 to 100 nucleotides. Therefore they have become a prerequisite for determining regions of interest for subsequent nucleotide sequencing. Special methods have been developed also for the analysis of protein-DNA interaction: e.g., direct visualization of specific protein-DNA complexes (enzymes, regulatory proteins), and analysis of structures with higher complexity (chromatin, transcription complexes).

References

Jan 1, 1978·Annual Review of Biophysics and Bioengineering·J D Griffith, G Christiansen
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R Lenhard-SchullerS Tonegawa
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P W EarlyL Hood
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E E MaxP Leder
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C Brack
Aug 1, 1979·Molecular & General Genetics : MGG·N E Murray, W S Kelley
Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·H J VollenweiderT Koller
Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S I ReedG R Stark
Sep 1, 1975·Journal of Virology·B Lescure, M Yaniv
Nov 24, 1975·Chromosoma·J E ManningN Davidson
Jan 1, 1975·Journal of Virology·C CremisiM Yaniv
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J E Mertz, J B Gurdon
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·M T Hsu, W R Jelinek
May 11, 1978·Nature·W FiersM Ysebaert
May 5, 1978·Science·V B ReddyS M Weissman
Aug 5, 1978·Journal of Molecular Biology·J O ThomasW Szer
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·O C RichardsJ Manning
Aug 10, 1979·Nucleic Acids Research·M WuN Davidson
Oct 1, 1979·Journal of Virology·M WuN Davidson
May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D D Brown, J B Gurdon
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S M BergetP A Sharp
Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S M TilghmanP Leder
Jan 1, 1978·Cold Spring Harbor Symposia on Quantitative Biology·T R BrokerB S Zain
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M F TrendelenburgJ B Gurdon
Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R C Williams
Sep 1, 1977·Analytical Biochemistry·M ZollingerM D Mamet-Bratley
Feb 1, 1978·Nucleic Acids Research·T R BrokerN Davidson
Aug 5, 1978·Journal of Molecular Biology·J O ThomasR Weisberg

❮ Previous
Next ❯

Citations

Jan 1, 1995·Micron : the International Research and Review Journal for Microscopy·E Kellenberger, H Wunderli-Allenspach
May 1, 1988·Quarterly Reviews of Biophysics·J DubochetP Schultz
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·B BlombergS Tonegawa
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J Lieman-HurwitzY Groner
Jan 1, 1982·The Journal of Experimental Medicine·Y Kurosawa, S Tonegawa
Feb 1, 1984·The Journal of Experimental Medicine·G HeinrichS Tonegawa
Nov 11, 1989·Nucleic Acids Research·E KönigI Roditi
Oct 1, 1998·Nucleic Acids Research·D V KlinovE D Sverdlov
Jun 29, 2004·Journal of Virology·Stefan HehnleHolger Jeske
Aug 1, 1991·Journal of Electron Microscopy Technique·E Le CamE Delain
Sep 25, 2010·Micron : the International Research and Review Journal for Microscopy·Yuri L Lyubchenko
Mar 1, 1992·Journal of Structural Biology·S Bratosin-Guttman
Jul 2, 1999·Biophysical Journal·L S ShlyakhtenkoY L Lyubchenko
Nov 15, 1984·Journal of Molecular Biology·A M GronenbornG M Clore
Apr 30, 1990·European Journal of Biochemistry·R WesselH Hoffmann-Berling
Sep 24, 1990·European Journal of Biochemistry·R WesselH Hoffmann-Berling
Mar 22, 2014·Soft Matter·Tatiana SchmatkoMounir Maaloum
Nov 1, 1988·Journal of Ultrastructure and Molecular Structure Research·C BrackL Brunner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.