PMID: 6770902Jun 27, 1980

DNA excision repair in human cells treated with ultraviolet radiation and 7,12-dimethylbenz[a]anthracene 5,6-oxide

Biochimica Et Biophysica Acta
F E AhmedR B Setlow

Abstract

Excision repair was measured in normal human and xeroderma pigmentosum group C cells treated with 7,12-dimethylbenz[a]-anthracene 5,6-oxide and with ultraviolet radiation by the techniques of unscheduled DNA synthesis, repair replication, a modification of bromodeoxyuridine photolysis employing the dye Hoechst 33258 and 365 nm radiation, and endonuclease-sensitive sites assay. Radioautography and repair replication showed that in normal cells the magnitude of repair after a saturation dose of epoxide (approx. 10 microM) to be 0.1-0.2 that after a saturating ultraviolet dose (20 J/m2 at 254), though survival data showed that both doses gave nearly similar killings. Repair was of the long-patch type and repair kinetics after the epoxide treatment were similar to ultraviolet. After a combined treatment with both agents, unscheduled synthesis in normal cells was more than additive, although, considering the experimental errors, these data and those of repair replication are consistent with additivity. The epoxide did not inhibit loss of sites sensitive to the ultraviolet endonuclease. However, after a combined treatment to xeroderma pigmentosum cells there was appreciably less unscheduled synthesis than for the sum of both treatmen...Continue Reading

References

Oct 4, 1976·Biochimica Et Biophysica Acta·C A Smith, P C Hanawalt
Jan 1, 1973·Proceedings of the Society for Experimental Biology and Medicine·H F Stich, R H San
Mar 18, 1969·Biochimica Et Biophysica Acta·C F Brunk, V Leick
Mar 1, 1970·Biophysical Journal·M M Elkind, C Kamper

Related Concepts

7,12-dimethylbenz(a)anthracene 5,6-oxide
Benz(a)Anthracenes
NSC-38297
Cell Survival
DNA, Double-Stranded
Base Excision Repair
DNA Replication
Epoxy Compounds
Fibroblasts
Photodegradation

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