DNA extraction using a tetramethyl orthosilicate-grafted photopolymerized monolithic solid phase

Analytical Chemistry
Jian WenJames P Landers

Abstract

A novel high-capacity, high-efficiency DNA extraction method is described using a photopolymerized silica-based monolithic column in a fused-silica capillary. Development involved investigation of the composition of the sol-gel monomer, fabrication conditions, and surface modifications in order to optimize the binding capacity. Extraction capacity and efficiency with the 3-(trimethoxysilyl)propyl methacrylate (TMSPM) monolith formulations fabricated in capillaries were investigated using a simple three-step procedure consisting of sample loading, washing of the solid phase, and elution of the DNA using a low ionic strength Tris buffer at pH 8. Once the TMSPM monomer concentration was optimized to yield a monolith with maximum test stability (robustness) and minimum back pressure, the monolith surface was modified by the grafting of tetramethyl orthosilicate (TMOS) for increased DNA binding capacity. After the examination of a variety of TMOS concentrations, 85% v/v TMOS was found to be optimal for DNA extraction without any obvious changes to the monolith structure. The reduction of time allowed for TMSPM hydrolysis prior to UV polymerization from 20 to 5 min led to a lower back pressure of the monolith, enabling better TMOS de...Continue Reading

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Citations

Aug 4, 2010·Analytical and Bioanalytical Chemistry·Cafer CakalPerihan Caglar
Sep 21, 2010·Analytical and Bioanalytical Chemistry·Li XuYu-Qi Feng
Sep 5, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hong Hanh TranNae Yoon Lee
Jan 11, 2011·Analytical Chemistry·Brian E RootAnnelise E Barron
Mar 11, 2010·Forensic Science International. Genetics·Joan M BienvenueJames P Landers
Jun 8, 2007·Journal of Forensic Sciences·Katie M HorsmanJames P Landers
May 13, 2015·Electrophoresis·Yong Tae KimTae Seok Seo
Jun 26, 2008·The Analyst·Silvija AbeleMirek Macka
Jul 4, 2009·Journal of Separation Science·Michal Szumski, Boguslaw Buszewski
Jul 9, 2009·Journal of Separation Science·Lijia LiuYibing Zhang
Oct 14, 2009·Lab on a Chip·Wenhua ZhangChaoyong James Yang
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Jungkyu KimBruce K Gale
Jan 30, 2008·The Analyst·Benjamin J HindsonJohn M Dzenitis
Aug 15, 2009·Lab on a Chip·Carol W PriceJames P Landers
Mar 12, 2011·Journal of Separation Science·Akira NameraShota Miyazaki
Aug 6, 2013·Analytical Methods : Advancing Methods and Applications·Samantha ByrnesCatherine M Klapperich
Mar 19, 2011·Analytica Chimica Acta·Cafer CakalPerihan Caglar
Mar 30, 2021·Emergent Materials·Claudia W Narvaez VillarrubiaGautam Gupta
May 7, 2010·Analytical Chemistry·Anirban ChatterjeeAlexis F Sauer-Budge
Aug 30, 2008·Analytical Chemistry·Jian WenJames P Landers
Jun 27, 2021·Science & Justice : Journal of the Forensic Science Society·Meghan MckinnonDenice Higgins
Sep 30, 2009·Analytica Chimica Acta·Kirsty J ShawStephen J Haswell
Nov 3, 2021·Journal of Separation Science·Mônica Silva AlvesJosias Merib

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