DNA Fingerprinting Analysis of Vegetative Compatibility Groups in Aspergillus flavus from a Peanut Field in Georgia

Plant Disease
Cesaria E McAlpinBruce W Horn

Abstract

The ability of species-specific DNA probe pAF28 to correctly match 75 strains of Aspergillus flavus isolated from a peanut field in Georgia with 1 of 44 distinct vegetative compatibility groupings (VCGs) was assessed. Multiple strains belonging to the same VCG typically produced identical DNA fingerprints, with the exception of VCG 17 and VCG 24, which contained strains that showed 83 and 87% similarity, respectively. A. flavus isolates sharing more than 80% of the fragments are recognized as belonging to the same DNA fingerprint group. Each VCG represented by a single isolate produced unique DNA fingerprints. The results provide further evidence that the pAF28 probe is able to distinguish A. flavus VCGs based on DNA fingerprints and can be used to predict the approximate number of VCGs in a sample population. The DNA probe also hybridized strongly and displayed multiple and distinct bands with other species in Aspergillus section Flavi: A. bombycis, A. caelatus, A. nomius, A. pseudotamarii, and A. tamarii. Although individual strains representing Aspergillus spp. in section Flavi produced DNA fingerprints with multiple bands, the banding patterns could not be used to classify these strains according to species.

References

Mar 1, 1995·Applied and Environmental Microbiology·C E McAlpin, B Mannarelli
Jan 1, 1993·Annual Review of Phytopathology·J F Leslie
Oct 1, 1998·Plant Disease·C E McAlpinC E Platis

❮ Previous
Next ❯

Citations

Sep 14, 2007·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·E Afriyie-GyawuT D Phillips
Feb 8, 2006·Canadian Journal of Microbiology·Cesaria E McAlpin, Donald T Wicklow
Feb 1, 2012·Mycotoxin Research·Sui Sheng T HuaSiov Bouy L Sarreal
Feb 21, 2008·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·T D PhillipsJ-S Wang
Mar 17, 2011·Phytopathology·Rebecca Ruth SweanyMichael Douglas Kaller
Sep 14, 2007·Food Additives and Contaminants·Bruce W Horn
Dec 13, 2012·Annals of the New York Academy of Sciences·Hillary L MehlPeter J Cotty
Mar 26, 2003·Applied Microbiology and Biotechnology·D BhatnagarT E Cleveland
Jun 24, 2008·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·E Afriyie-GyawuT D Phillips

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.