PMID: 8582346Sep 1, 1995Paper

DNA fingerprinting to determine paternity in laboratory rat sperm competition experiments

Electrophoresis
G A ShimminP D Temple-Smith

Abstract

Prior to this study a significant amount of research had been undertaken in the field of sperm competition in mammals. However, males of different strains have been required in each of these studies to enable paternity assignment through gene expression, which has consequently resulted in problems with differential fertilising capacity being encountered. In this study paternity assignment of progeny from sperm competition experiments with Sprague Dawley rats was achieved by multilocus DNA fingerprinting using band locus matching of individual specific banding patterns between progeny and parents. Trials with 4 restriction enzymes and 5 digoxygenin labelled probes (4 oligonucleotide and 1 cloned) achieved the highest levels of DNA fingerprint heterozygosity using AluI(CAC)5 and HinfI(CAC)5 combinations; however, paternity could not be determined in all offspring, due to a higher than expected degree of inbreeding within the rat population used in this study. This was demonstrated in subsequent comparisons of genetic diversity of three laboratory rat breeding populations from two different animal breeding facilities. Data from the rat mating study showed that, under conditions of direct sperm competition, second males given acces...Continue Reading

References

Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N Yuhki, S J O'Brien
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H K ReeveC F Aquadro
Feb 11, 1988·Nucleic Acids Research·S A MillerH F Polesky
Jun 1, 1972·Journal of Reproduction and Fertility·A Tsafriri, P F Kraicer
Sep 1, 1982·Irish Journal of Medical Science·D J CarsonN A Carson

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Citations

Oct 1, 2008·Physiology & Behavior·Maha Zewail-FooteFay A Guarraci
Dec 21, 2006·Physiology & Behavior·Jennifer L LovellFay A Guarraci

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