PMID: 6987317Mar 1, 1980Paper

DNA flow cytometry on human epidermis: I. methodological studies on normal skin

The Journal of Investigative Dermatology
G FrentzI Christensen

Abstract

A method for single cell DNA measurements by flow cytometry based on a technique successfully used for the hamster cheek pouch epithelium was extended for studies on human epidermis. A least squares estimation procedure was used for the statistical analysis of the initially obtained DNA frequency distribution. In 14 specimens of abdominal skin from a young healthy female the precentages of epidermal cells with an S-phase DNA content were 3.0 +/- 0.6 (SD), and the percentages of epidermal cells with a G2+M phase DNA content 1.1 +/- 0.1 (SD). No significant differences in values were found for specimens investigated in different experiments. No influence of tissue storage time for a maximum of 32 days in liquid nitrogen was discernible. Values from the corresponding dermal tissue were significantly more variable then the epidermal values, but the levels were of the same magnitude. It is concluded that this technique used for human epidermis yields sufficient stable and accurate estimates of S and G2+M fractions that it should be a valuable tool useful as an alternative or a supplement to conventional cell kinetic methods.

Citations

Jan 1, 1985·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·G FrentzJ K Larsen
Jan 1, 1983·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·P K JensenL Bolund
Jul 1, 1982·The British Journal of Dermatology·G FrentzN Keiding
Sep 1, 1984·The British Journal of Dermatology·G Frentz, U Møller
Feb 1, 1983·Journal of Cutaneous Pathology·O P Clausen
Oct 1, 1992·Journal of Cutaneous Pathology·S T NedorostM G Fleming
Jan 1, 1985·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·J K LarsenI J Christensen
Jan 1, 1988·Archives of Dermatological Research·F W Bauer, J B Boezeman
Jul 1, 1981·Cytometry·O D Laerum, T Farsund
Jan 1, 1983·Clinical and Experimental Dermatology·G Frentz, U Møller
Jan 1, 1983·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·K SøndergaardK Hou-Jensen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.