DNA fragment sizing by single molecule detection in submicrometer-sized closed fluidic channels

Analytical Chemistry
M FoquetH G Craighead

Abstract

The fabrication of fluidic channels with dimensions smaller than 1 microm is described and characterized in respect to their use for detection of individual DNA molecules. The sacrificial layer technique is used to fabricate these devices as it provides CMOS-compatible materials exhibiting low fluorescence background. It also allows creating microfluidics circuitry of submicrometer dimensions with great control. The small dimensions facilitate single molecule detection and minimize events of simultaneous passage of more than one molecule through the measurement volume. The behavior of DNA molecules inside these channels under an applied electrical field was first studied by fluorescence correlation spectroscopy using M13 double-stranded DNA. A linear relationship between the flow speed and applied electric field across the channel was observed. Speeds as high as 5 mm/s were reached, corresponding to only a few milliseconds of analysis time per molecule. The channels were then used to characterize a mixture of nine DNA fragments. Both the distribution and relative proportions of the individual fragments, as well as the overall concentration of the DNA sample, can be deduced from a single experiment. The amount of sample required...Continue Reading

References

Feb 25, 1993·Nucleic Acids Research·P M GoodwinR A Keller
Feb 3, 2000·Analytical Chemistry·A Van OrdenW P Ambrose
Nov 25, 2000·Science·H G Craighead
Mar 21, 2001·Journal of Biotechnology·K DörreM Eigen
Feb 29, 2008·Applied Optics·A AgronskaiaJ Greve

❮ Previous
Next ❯

Citations

Aug 3, 2005·Analytical and Bioanalytical Chemistry·Petra S Dittrich, Andreas Manz
Mar 15, 2008·Analytical and Bioanalytical Chemistry·Nicholas DouvilleShuichi Takayama
Oct 7, 2008·Analytical and Bioanalytical Chemistry·Daniel J Eves, Adam T Woolley
Sep 10, 2010·Analytical and Bioanalytical Chemistry·Christopher A BakerMichael G Roper
Apr 15, 2005·Mutation Research·Eugene Y Chan
Sep 28, 2002·Journal of Chromatography. a·J Gaudioso, H G Craighead
Oct 11, 2003·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Katri HuikkoTapio Kotiaho
Dec 14, 2012·Analytical Chemistry·Laurent D Menard, J Michael Ramsey
Feb 27, 2010·Analytical Chemistry·Benjamin R CiprianyPaul D Soloway
Apr 7, 2010·Journal of the American Chemical Society·Kelvin J LiuTza-Huei Wang
May 12, 2012·The Journal of Physical Chemistry. B·Perry G SchiroDaniel T Chiu
Oct 11, 2008·Nano Letters·Stephen L LevyHarold G Craighead
Mar 16, 2007·Chemical Communications : Chem Comm·A HuebnerJ B Edel
Feb 2, 2011·Chemical Communications : Chem Comm·Rohan T Ranasinghe, Tom Brown
Jun 18, 2005·Proceedings of the National Academy of Sciences of the United States of America·Michal TokarzOwe Orwar
May 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·Benjamin R CiprianyHarold G Craighead
Dec 19, 2006·Nucleic Acids Research·Ming XiaoPui-Yan Kwok
May 8, 2004·Journal of Clinical Microbiology·Matthew M FerrisBabetta L Marrone
May 1, 2004·Annual Review of Physical Chemistry·Mattias KarlssonOwe Orwar
Apr 2, 2004·Journal of Nanobiotechnology·Richard Barry, Dimitri Ivanov
Feb 18, 2010·International Journal of Molecular Sciences·Jérome Wenger, Hervé Rigneault
Aug 17, 2012·Medical Physics·Tian-Cai LiuLi-Ping Wang
Jul 8, 2008·Journal of Biomedical Optics·Cheng Frank ZhongTheodore B Norris
Sep 29, 2012·Lab on a Chip·Jaime J BenítezHarold G Craighead
Jul 1, 2008·Methods : a Companion to Methods in Enzymology·Jose M Moran-Mirabal, Harold G Craighead
Mar 15, 2008·Biophysical Journal·Christian H RecciusH G Craighead
Jan 18, 2006·Current Opinion in Biotechnology·Mona Wells
May 17, 2005·Biophysical Journal·Joseph P SkinnerJoachim D Müller
Aug 29, 2003·Biophysical Journal·Lindsey N Hillesheim, Joachim D Müller
Dec 25, 2008·Lab on a Chip·Danny BottenusCornelius F Ivory
Jul 19, 2005·Journal of Colloid and Interface Science·Anpan HanUrs Staufer
Aug 4, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Robert C Habbersett, James H Jett

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.