DNA fragmentation, an indicator of apoptosis, in cultured black tiger shrimp Penaeus monodon infected with white spot syndrome virus (WSSV)

Diseases of Aquatic Organisms
A H SahtoutM Shariff

Abstract

Fifty black tiger shrimp Penaeus monodon from commercial cultivation ponds in Malaysia were examined by Tdt-mediated dUTP nick-end labeling (TUNEL) fluorescence assay and agarose gel electrophoresis of DNA extracts for evidence of DNA fragmentation as an indicator of apoptosis. From these specimens, 30 were grossly normal and 20 showed gross signs of white spot syndrome virus (WSSV) infection. Of the 30 grossly normal shrimp, 5 specimens were found to be positive for WSSV infection by normal histology and by nested polymerase chain reaction (PCR) analysis. All of the specimens showing gross signs of WSSV infection were positive for WSSV by normal histology, while 5 were positive by nested PCR only (indicating light infections) and 15 were positive by 1-step PCR (indicating heavy infections). Typical histological signs of WSSV infection included nuclear hypertrophy, chromatin condensation and margination. None of the 25 grossly normal shrimp negative for WSSV by 1-step PCR showed any signs of DNA fragmentation by TUNEL assay or agarose gel electrophoresis of DNA extracts. The 10 specimens that gave PCR-positive results for WSSV by nested PCR only (i.e., 5 grossly normal shrimp and 5 grossly positive for WSSV) gave mean counts of...Continue Reading

Citations

Apr 16, 2010·Marine Biotechnology·T W Flegel, Kallaya Sritunyalucksana
Mar 13, 2014·Journal of Invertebrate Pathology·Fang-fang MaJie Huang
Oct 18, 2012·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Jian-An XianAn-Li Wang
Jun 12, 2012·Fish & Shellfish Immunology·Jiann-Horng LeuChu-Fang Lo
Dec 8, 2015·Journal of Zhejiang University. Science. B·Hai-sheng XuYu-yin Chen
Mar 3, 2011·Fish & Shellfish Immunology·Huali ZuoWei Wang
May 18, 2010·Journal of Virological Methods·Grit MrotzekHans P Saluz
Feb 19, 2009·Fish & Shellfish Immunology·Haipeng LiuPikul Jiravanichpaisal
Aug 6, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yusu XieJianhai Xiang
Oct 2, 2007·Developmental and Comparative Immunology·Jiann-Horng LeuChu-Fang Lo
Oct 20, 2006·Fish & Shellfish Immunology·Sudkhate MolthathongTimothy W Flegel
Sep 27, 2014·The Journal of Parasitology·Reginald B Blaylock, Stephen A Bullard
Jun 22, 2016·Fish & Shellfish Immunology·Anchulee SaleeartPongsak Khunrae
Aug 27, 2016·Fish & Shellfish Immunology·Kwanta ThansaHathaitip Suksodsai
Aug 30, 2008·Fish & Shellfish Immunology·Wei Wang, Xiaobo Zhang
Feb 26, 2010·Veterinary Research·Arturo Sánchez-Paz
Oct 3, 2018·Journal of Fish Diseases·Chen-Fei LowNur Farahiyah Ahmad Nadzri
Jul 7, 2018·Clinical Microbiology Reviews·Naveen KumarBarry T Rouse
Sep 16, 2020·Journal of Fish Diseases·Jeff A Cowley

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis