DNA fragmentation is a feature of cystic fibrosis epithelial cells: a disease with inappropriate apoptosis?

FEBS Letters
L MaiuriS Auricchio

Abstract

Cystic fibrosis (CF) is a single-gene disease caused by mutations in the CFTR gene, which result in disrupted chloride secretions with inspissated mucous secretions by exocrine glands. Nick-end labelling was used to assess DNA fragmentation in 14 CF and 24 control duodenal samples, and in two CF and two control lung tissues. In CF small intestine median 46% (range: 30-82) villus enterocytes show DNA fragmentation (vs. 3% (range: 1-7) in controls P < 0.001) and median 37.5% (range: 23-79) crypt enterocytes show Ki67 antigen (P < 0.001). In CF airways 57% (range: 54-70) of epithelial cells show DNA fragmentation. Inappropriate high DNA fragmentation is a feature of various CF epithelia. This could have great impact in understanding the mechanisms leading to disease.

References

Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Jan 1, 1982·Journal of Pediatric Gastroenterology and Nutrition·P KuitunenE Savilahti
Jan 1, 1995·Journal of Clinical Immunology·J D MountzJ Cheng
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·R A GottliebB M Babior
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·M A ValverdeW H Colledge
Aug 1, 1995·Experimental Cell Research·B Della GasperaD C Gruenert
Jan 1, 1995·Acta Neuropathologica·H LassmannH M Wisniewski
Dec 16, 1994·Science·L H Hartwell, M B Kastan
Apr 1, 1994·The Journal of Experimental Medicine·E M Tan
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·R A Gottlieb, A Dosanjh
Apr 1, 1996·Nature Genetics·X Estivill
Aug 1, 1996·Gastroenterology·S F Moss, P R Holt

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Citations

May 8, 2010·Apoptosis : an International Journal on Programmed Cell Death·Olga KryskoClaus Bachert
Jun 25, 2013·Apoptosis : an International Journal on Programmed Cell Death·Raffaella SoletiMaria Carmen Martínez
Aug 1, 2009·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Malgorzata Mrugacz
May 9, 2000·Archives of Disease in Childhood·R L SmythA Ferguson
Jun 5, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Matthew J DiMagnoShi-yi Zhou
Mar 8, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Manish BodasNeeraj Vij
Mar 6, 2002·The Journal of Clinical Investigation·R William VandivierPeter M Henson
Mar 17, 2009·Respiratory Research·Mathilde RottnerM Carmen Martínez
Jun 27, 2002·American Journal of Pharmacogenomics : Genomics-related Research in Drug Development and Clinical Practice·O EidelmanH B Pollard
Jul 8, 2014·Apoptosis : an International Journal on Programmed Cell Death·Jia-Wei ZengYong-Yuan Guan
Jan 20, 2010·Archives of Medical Research·Ebru YalçinNural Kiper
Nov 17, 2010·Biochemical and Biophysical Research Communications·Katrin Anne BeckerHeike Grassmé
Oct 22, 2013·Biochimica Et Biophysica Acta·Anna CarettiPaola Signorelli
Apr 20, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mathilde RottnerMaría Carmen Martínez
Nov 13, 2009·American Journal of Physiology. Renal Physiology·Sebastien l'HostePhilippe Poujeol
Aug 15, 2001·American Journal of Physiology. Cell Physiology·H BarrièreP Poujeol
Dec 12, 2002·American Journal of Physiology. Renal Physiology·Hervé BarrièrePhilippe Poujeol

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