Apr 12, 2016

DNA from fecal immunochemical test can replace stool for microbiota-based colorectal cancer screening

BioRxiv : the Preprint Server for Biology
Nielson T BaxterPatrick D Schloss

Abstract

Background: There is a significant demand for colorectal cancer (CRC) screening methods that are noninvasive, inexpensive, and capable of accurately detecting early stage tumors. It has been shown that models based on the gut microbiota can complement the fecal occult blood test and fecal immunochemical test (FIT). However, a barrier to microbiota-based screening is the need to collect and store a patient's stool sample. Methods: Using stool samples collected from 404 patients we tested whether the residual buffer containing resuspended feces in FIT cartridges could be used in place of intact stool samples. Results: We found that the bacterial DNA isolated from FIT cartridges largely recapitulated the community structure and membership of patients' stool microbiota and that the abundance of bacteria associated with CRC were conserved. We also found that models for detecting CRC that were generated using bacterial abundances from FIT cartridges were equally predictive as models generated using bacterial abundances from stool. Conclusions: These findings demonstrate the potential for using residual buffer from FIT cartridges in place of stool for microbiota-based screening for CRC. This may reduce the need to collect and process ...Continue Reading

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Mentioned in this Paper

Biological Markers
Buffers
Lymphoma Stage I
Neoplasms
Fecal Immunochemical Test
Noninvasive
Microbiome
Colorectal Cancer Pathway
Microbiota (Procedure)
Cartridge Device

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