Abstract
We have designed DNA fusion vaccines able to induce high levels of epitope-specific CD8(+) T cells, using linked CD4(+) T cell help. Such vaccines can activate effective immunity against tumor Ags. To model performance against minor histocompatibility (H) Ags important in allogeneic hemopoietic stem cell transplantation, responses against the H2D(b)-restricted Uty and Smcy male HY epitopes have been investigated. Vaccination of females induced high levels of tetramer-specific, IFN-gamma-producing CD8(+) T cells against each epitope. Vaccines incorporating a single epitope primed effector CTL able to kill male splenocytes in vitro and in vivo, and HY(Db)Uty-specific vaccination accelerated rejection of syngeneic male skin grafts. Priming against either epitope established long-term memory, expandable by injection of male cells. Expanded CD8(+) T cells remained specific for the priming HY epitope, with responses to the second suppressed. To investigate vaccine performance in a tolerized repertoire, male mice were vaccinated with the fusion constructs. Strikingly, this also generated epitope-specific IFN-gamma-producing CD8(+) T cells with cytotoxic function. However, numbers and avidity were lower than in vaccinated females, and ...Continue Reading
References
Feb 1, 1992·European Journal of Immunology·Y Uematsu
Aug 1, 1991·Immunological Reviews·G J HämmerlingB Arnold
Mar 25, 1988·Cell·Y UematsuM Steinmetz
Jun 23, 1988·Nature·P KisielowH von Boehmer
Jun 1, 1988·Annals of Internal Medicine·J M GoldmanP B McGlave
Feb 1, 1995·International Immunology·J AlferinkB Arnold
May 1, 1993·The Journal of Surgical Research·H BassiriC F Barker
May 1, 1996·Nature Medicine·H E HeslopC M Rooney
Oct 4, 1996·Science·J D AltmanM M Davis
Dec 1, 1996·Nature Genetics·A GreenfieldP Koopman
Jun 1, 1997·Vaccine·S A Johnston, M A Barry
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·M A MarkiewiczP G Ashton-Rickardt
May 16, 1998·European Journal of Immunology·H L SnyderJ R Bennink
Jun 10, 1998·Immunity·C TanchotB Rocha
Nov 11, 1998·Nature Medicine·C A KingF K Stevenson
Oct 12, 1999·Experimental Hematology·F DazziJ M Goldman
Feb 13, 2001·Immunity·C BouneaudP Bousso
Jul 4, 2001·Nature Medicine·F DazziJ M Goldman
Jul 4, 2001·Nature Medicine·P FontaineC Perreault
Jul 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·J RiceF K Stevenson
Sep 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M MillrainP J Dyson
Nov 28, 2001·Immunological Reviews·D ZelenikaS P Cobbold
Dec 26, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rana A K SinghMichael A Barry
Mar 8, 2002·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·E SimpsonP J Dyson
Apr 3, 2002·Nature Medicine·Anne M DickinsonEls Goulmy
May 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Monika C WolkersTon N M Schumacher
Sep 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jason RiceFreda K Stevenson
Nov 5, 2002·Nature Immunology·Chun-Yen LinHerman Waldmann
Jul 19, 2003·Blood·Mirjam H M HeemskerkJ H Frederik Falkenburg
Feb 21, 2004·Advances in Immunology·Freda K StevensonDelin Zhu
Citations
May 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sarah BuchanJason Rice
Oct 15, 2005·Current Opinion in Oncology·Freda K Stevenson
Dec 14, 2006·Blood·Gregory KanterRonald Levy
Sep 5, 2006·The Journal of Biological Chemistry·Hester A DoyleMark J Mamula
Feb 21, 2007·Scandinavian Journal of Immunology·L SteinaaA Gautam
Apr 6, 2007·Expert Review of Vaccines·Shaw-Wei D TsenT-C Wu
Sep 24, 2010·Leukemia·D Joseph-PietrasS S Sahota
Mar 16, 2013·International Journal of Cancer. Journal International Du Cancer·Juan Campos-PerezFreda K Stevenson
Sep 19, 2006·Bone Marrow Transplantation·R MunkerH J Kolb
May 20, 2008·International Immunology·Hiroyasu ItoMitsuru Seishima
Mar 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nathan J RobertsonJulian Dyson
May 27, 2008·Blood·Coralie ChaiseFreda K Stevenson
Jul 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael J PalmowskiVincenzo Cerundolo
Apr 28, 2006·European Journal of Immunology·Freda K Stevenson, Jason Rice
Jan 8, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna L FurmanskiJulian Dyson
Jul 16, 2008·European Journal of Immunology·Jason RicePhilip D Greenberg
May 24, 2011·European Journal of Immunology·Gisella E VittesFreda K Stevenson
Jan 26, 2008·Nature Reviews. Cancer·Jason RiceFreda K Stevenson
Apr 29, 2014·European Journal of Immunology·Adam E SnookScott A Waldman
Jun 7, 2011·Cancer Immunology, Immunotherapy : CII·Freda K StevensonChristian H Ottensmeier
May 19, 2006·Cancer Research·Jason RiceFreda K Stevenson