PMID: 11909665Mar 23, 2002Paper

DNA genotyping of unbuffered formalin fixed paraffin embedded tissues

Forensic Science International : Synergy
Beatrice LegrandKarine Crainic

Abstract

Formalin-induced DNA degradation was studied at different fixation times (3, 7, 16 and 32 days) each on 10 formalin fixed paraffin embedded tissues (FFPET) stored for 15 years at room temperature. The four different extraction protocols used in this study showed that Chelex100 extracts performed the best at 3 and 7 days of formalin fixation (DFF) (with regard to the quantity and the quality of the DNA). However, Qiamp extracts showed better results for long sized alleles, as well for single polymerase chain reaction (PCR) amplifications after 16 and 32 DFF, as for multiplex PCR at shorter fixation times. DNA degradation is expressed by the size of the amplified alleles, only 100 bp templates surviving after 32 DFF (AMG locus). Single locus amplifications (CD4 and FES/FPS alleles) performed better than multiplex PCR (ProfilerPlus), with nearly 100% positive results at 7 DFF. In both types of amplifications, the success rate decreased proportionally with the time of formalin fixation and, consequently, with the size of the required DNA template.

References

Jan 1, 1992·International Journal of Legal Medicine·W Huckenbeck, W Bonte
Jul 25, 1991·Nucleic Acids Research·M H PolymeropoulosC R Merril
Feb 1, 1991·The American Journal of Surgical Pathology·C MiesR S Chaganti
Sep 1, 1990·Journal of Clinical Pathology·Y TokudaT Sugiyama
Jul 16, 1985·Biochemical and Biophysical Research Communications·S E GoelzB Vogelstein
May 1, 1996·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·N YagiS Maeda
Sep 1, 1996·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·T S FrankE D Hsi
Jan 23, 1999·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·T MizunoT Seyama
Aug 30, 2000·Virchows Archiv : an International Journal of Pathology·A D CabrasH Höfler
Sep 9, 2000·Forensic Science International : Synergy·S BanaschakB Brinkmann

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Citations

Jun 2, 2005·Trends in Biotechnology·Fabrice TeletcheaCatherine Hänni
Mar 12, 2004·Molecular Phylogenetics and Evolution·A Bucklin, L D Allen
Jun 29, 2006·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·J EdelmannL-C Horn
Dec 15, 2006·The British Journal of Ophthalmology·Nicolai Christian SjöSteffen Heegaard
Nov 4, 2015·Frontiers in Microbiology·Paul B HamiltonRoger D Bull
Aug 3, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Marc BrulportJan G Hengstler
May 14, 2011·Molecular Ecology Resources·J Zhang
Apr 9, 2014·Journal of Forensic Sciences·Marc L SprouseRhonda K Roby
Feb 8, 2013·International Journal of Cancer. Journal International Du Cancer·Eugen DhimoleaPeter Geck
Dec 8, 2005·British Journal of Cancer·E H van BeersP M Nederlof
Mar 14, 2018·The American Journal of Dermatopathology·Amanda B MoyerPatricia Chévez-Barrios
Nov 30, 2006·The American Journal of Forensic Medicine and Pathology·Masao OtaHirofumi Fukushima
Jun 27, 2008·Advances in Anatomic Pathology·Jennifer L Hunt
May 20, 2020·Journal of Anatomy·Ahlam AlqahtaniBill Chaudhry
May 22, 2019·Electrophoresis·Concetta CafieroCristina Grippaudo
Aug 30, 2003·Analytical Chemistry·T A BrettellR Saferstein
Feb 7, 2008·Archives of Pathology & Laboratory Medicine·Jennifer L Hunt
Feb 22, 2011·Science & Justice : Journal of the Forensic Science Society·Ayeleth ReshefRoni Gafny

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