DNA gyrase is involved in chloroplast nucleoid partitioning

The Plant Cell
Hye Sun ChoHyun-Sook Pai

Abstract

DNA gyrase, which catalyzes topological transformation of DNA, plays an essential role in replication and transcription in prokaryotes. Virus-induced gene silencing of NbGyrA or NbGyrB, which putatively encode DNA gyrase subunits A and B, respectively, resulted in leaf yellowing phenotypes in Nicotiana benthamiana. NbGyrA and NbGyrB complemented the gyrA and gyrB temperature-sensitive mutations of Escherichia coli, respectively, which indicates that the plant and bacterial subunits are functionally similar. NbGyrA and NbGyrB were targeted to both chloroplasts and mitochondria, and depletion of these subunits affected both organelles by reducing chloroplast numbers and inducing morphological and physiological abnormalities in both organelles. Flow cytometry analysis revealed that the average DNA content in the affected chloroplasts and mitochondria was significantly higher than in the control organelles. Furthermore, 4',6-diamidino-2-phenylindole staining revealed that the abnormal chloroplasts contained one or a few large nucleoids instead of multiple small nucleoids dispersed throughout the stroma. Pulse-field gel electrophoresis analyses of chloroplasts demonstrated that the sizes and/or structure of the DNA molecules in the ...Continue Reading

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Citations

Apr 9, 2008·Plant Cell Reports·Jasmina Rokov-PlavecIvana Weygand-Durasevic
Sep 25, 2012·Planta·Chang Sook AhnHyun-Sook Pai
Feb 14, 2009·Molecular Genetics and Genomics : MGG·Carolina V MorganteMarcio C Silva-Filho
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Apr 28, 2006·The Plant Journal : for Cell and Molecular Biology·Chang Sook AhnHyun-Sook Pai
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