DNA-hosted copper nanoclusters/graphene oxide based fluorescent biosensor for protein kinase activity detection

Analytica Chimica Acta
Mengke WangXingguang Su

Abstract

A novel fluorescent biosensor for protein kinase activity (PKA) detection was designed by applying double-strands DNA-hosted copper nanoclusters (dsDNA-CuNCs) and graphene oxide (GO). One DNA strand of the dsDNA consisted of two domains, one domain can hybridize with another complementary DNA strand to stabilize the fluorescent CuNCs and another domain was adenosine 5'-triphosphate (ATP) aptamer. ATP aptamer of the dsDNA-CuNCs would be spontaneously absorbed onto the GO surface through π-π stacking interactions. Thus GO can efficiently quench the fluorescence (FL) of dsDNA-CuNCs through fluorescence resonance energy transfer (FRET). In the present of ATP, ATP specifically combined with ATP aptamer to form ATP-ATP aptamer binding complexes, which had much less affinity to GO, resulting in the fluorescence recovery of the system. Nevertheless, in the presence of PKA, ATP could be translated into ADP and ADP could not combine with ATP aptamer resulting in the fluorescence quenching of dsDNA-CuNCs again. According to the change of the fluorescence signal, PKA activity could be successfully monitored in the range of 0.1-5.0 U mL-1 with a detection limit (LOD) of 0.039 U mL-1. Besides, the inhibitory effect of H-89 on PKA activity wa...Continue Reading

Citations

Jul 13, 2019·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Chung-An ChenKevin C-W Wu
Apr 22, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shan ZhangZhiqiang Su
Mar 25, 2019·International Journal of Molecular Sciences·Zhiyong YanYang Liu
Aug 28, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xinrui LiuRen-Jei Chung
Apr 20, 2021·Analytical Methods : Advancing Methods and Applications·Xiaojuan AiChangbei Ma
Jun 30, 2019·Analytical Chemistry·Rui LiuYi Lv
Mar 7, 2020·The Journal of Physical Chemistry Letters·Xuejuan ZouManzhou Zhu

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