PMID: 2109086Mar 1, 1990Paper

DNA hybridization as a guide to phylogeny: chemical and physical limits

Journal of Molecular Evolution
C W Schmid, J Marks

Abstract

The technique of forming interspecific DNA heteroduplexes and estimating phylogenetic distances from the depression in their duplex melting temperature has several physical and chemical constraints. These constraints determine the maximum phylogenetic distance that may be estimated by this technique and the most appropriate method of analyzing that distance. Melting curves of self-renatured single copy primate DNAs reveal the presence of components absent from the renaturation products of exactly paired sequences. This observation, which confirms existing literature, challenges a fundamental assumption: that orthologous (i.e., corresponding) DNA sequences in the divergent species are being compared in DNA heteroduplex melting experiments. As a model system, the thermal stabilities of heteroduplexes formed between a human alpha-globin cDNA and four alpha-like globin genes isolated from chimpanzee are qualitatively compared. The results of this comparison show that the cross-hybrids of imperfectly matched gene duplicates from divergent species can contribute to the additional components that are present in renatured single copy DNAs. Single copy DNA, as usually defined, includes sequence duplicates that will obscure phylogenetic ...Continue Reading

References

Jun 16, 1978·Molecular Biology Reports·I IvanovG Markov
Jun 28, 1978·Molecular and Cellular Biochemistry·I G Ivanov, G G Markov
Oct 1, 1989·Journal of Molecular Evolution·F H Sheldon, A H Bledsoe
Jan 1, 1986·Cold Spring Harbor Symposia on Quantitative Biology·J MarksC K Shen
Jan 1, 1985·Journal of Molecular Evolution·C WillardC W Schmid
Jan 1, 1987·Journal of Molecular Evolution·C G Sibley, J E Ahlquist
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J MarksC K Shen
Dec 20, 1986·Journal of Molecular Biology·I Sawada, C W Schmid
Feb 25, 1986·Nucleic Acids Research·R C HardisonC W Schmid
Oct 15, 1974·FEBS Letters·I G Ivanov, G G Markov
Dec 5, 1973·Journal of Molecular Biology·T I BonnerR J Britten
Jun 1, 1971·The Quarterly Review of Biology·R J Britten, E H Davidson
Feb 14, 1968·Journal of Molecular Biology·J G Wetmur, N Davidson
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J F HessC K Shen
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Dec 20, 1983·Nucleic Acids Research·S A Liebhaber, K A Begley
Dec 1, 1980·Gene·C CavallescoS M Weissman
Oct 17, 1980·Biochimica Et Biophysica Acta·G M Fox, C W Schmid
Oct 17, 1980·Biochimica Et Biophysica Acta·G M FoxC W Schmid

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Citations

Jun 1, 1991·American Journal of Physical Anthropology·J Marks
May 1, 1990·Journal of Molecular Evolution·A H Bledsoe, F H Sheldon
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J A KirschM S Springer
Nov 12, 2013·Journal of the History of Biology·Edna Suárez-Díaz
Oct 24, 2001·Trends in Genetics : TIG·J G Hacia
May 1, 1994·American Journal of Physical Anthropology·J Marks
Dec 4, 2016·Journal of Molecular Evolution·Edna Suárez-Díaz

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