DNA hybridization biosensors using polylysine modified SPCEs.

Biosensors & Bioelectronics
María Díaz-GonzálezAgustín Costa-García

Abstract

Two electrochemical DNA hybridization biosensors (genosensors) for the detection of a 30-mer sequence unique to severe acute respiratory syndrome (SARS) virus are described in this work. Both genosensors rely on the hybridization of the oligonucleotide target with its complementary probe, which is immobilized on positively charged polylysine modified screen-printed carbon electrodes (SPCEs), through electrostatic interactions. In one design, a biotinylated target is used and the detection of the hybridization reaction is monitored using alkaline phosphatase labeled streptavidin (S-AP). This enzyme catalyzes the hydrolysis of the substrate 3-indoxyl phosphate (3-IP) to indigo, which is then solubilized to indigo carmine and detected by means of cyclic voltammetry (CV). In the other design, the target is labeled using an Au(I) complex, sodium aurothiomalate, and the duplex formation is detected by measuring, for first time, the current generated by the hydrogen evolution catalyzed by the gold label. Using 30 min of hybridization time, a detection limit of 8 pM is calculated for the enzymatic genosensor. Although this good sensitivity cannot be reached with the metal label (0.5 nM), the use of this label allows a considerable decr...Continue Reading

Citations

Feb 4, 2012·ACS Applied Materials & Interfaces·Ahmed AiedWenxin Wang
Sep 29, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Ehsan ShabaniMohammad J Abdekhodaie
Jul 1, 2018·Electroanalysis·Filiz KuralayYaşar Bayramlı
Mar 21, 2020·Analytical and Bioanalytical Chemistry·Eka NovianaCharles S Henry
Nov 17, 2009·Analytical Chemistry·Alfredo de la Escosura-MuñizArben Merkoçi
Dec 6, 2012·Analytical Chemistry·Elaheh FarjamiElena E Ferapontova
Sep 28, 2010·Analytica Chimica Acta·A Bonanni, M del Valle

❮ Previous
Next ❯

Methods Mentioned

BETA
genosensor
biosensors
genosensors

Software Mentioned

Autolab GPES

Related Concepts

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.