PMID: 16507410Mar 2, 2006Paper

DNA hypermethylation of TIMP3 gene in invasive breast ductal carcinoma

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
E L H LuiL W C Chow

Abstract

Neoplastic cells often display aberrant methylation and silencing of multiple genes, including tumor suppressor genes (TSGs) that regulate critical processes such as cell cycle control, DNA repair and angiogenesis. Tissue inhibitor of metalloproteinase-3 (TIMP3) is an extracellular matrix-bound protein which regulates matrix composition and affects tumor growth, invasion and angiogenesis. It mediates vascular endothelial growth factor (VEGF) by blocking the binding of VEGF to VEGF receptor-2 and inhibits downstream signaling. This study focused on the hypermethylation status of the TIMP3 gene with clinical parameters in invasive breast ductal carcinoma (IDC) samples. DNA extraction and methylation specific PCR (MSP) was performed on 173 patients with invasive breast carcinoma. Both specific methylated and unmethylated primers for each gene were used for PCR and the products were visualized on agarose gel. The methylation status of TIMP3 was then compared with corresponding patients' clinicopathologic characteristics. Methylation frequencies of TIMP3 in the breast cancer samples were 20.81 %. Among the hypermethylated cancers, 50% were tumor grade II-III, 44.44% were positive in lymph node involvement (LN), 36.11% were positive ...Continue Reading

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Citations

Apr 4, 2008·Clinical & Experimental Metastasis·David I Rodenhiser
Nov 5, 2010·Journal of Translational Medicine·Wings T Y LooLouis W C Chow
Jan 12, 2008·World Journal of Gastroenterology : WJG·Eric SmithPaul A Drew
Sep 27, 2013·Carcinogenesis·Kathleen L Decicco-SkinnerJonathan S Wiest
Jun 19, 2007·The Journal of Steroid Biochemistry and Molecular Biology·Ming ZhangTian-Hui Zhu
Jan 27, 2015·Translational Research : the Journal of Laboratory and Clinical Medicine·Ivana FridrichovaLudovit Danihel
Mar 3, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Damien MassonMarc G Denis
Mar 17, 2011·The American Journal of Pathology·Hai-Xing JuYutaka Kondo
Mar 11, 2018·Pathology Oncology Research : POR·Ivana Maleva KostovskaDijana Plaseska-Karanfilska
Apr 7, 2007·International Journal of Cancer. Journal International Du Cancer·Beatrix VersmoldAndreas Waha
Oct 1, 2019·Journal of Cellular and Molecular Medicine·María Auxiliadora Olivares-UrbanoMaría Isabel Núñez
Nov 7, 2019·British Journal of Pharmacology·Micah G DonovanDonato F Romagnolo

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