DNA hypomethylation circuit of the mouse oocyte-specific histone H1foo gene in female germ cell lineage

Biology of Reproduction
Chiaki MaedaKunio Shiota

Abstract

The oocyte-specific subtype of the linker histone H1 is H1FOO, which constitutes a major part of oocyte chromatin. H1foo is expressed in growing oocytes, through fertilization, up until the two-cell embryo stage, when it is subsequently replaced by somatic H1 subtypes. To elucidate whether an epigenetic mechanism is involved in the limited expression of H1foo, we analyzed the dynamics of the DNA methylation status of the H1foo locus in germ and somatic cells. We identified a tissue-dependent and differentially methylated region (T-DMR) upstream of the H1foo gene, which was hypermethylated in sperm, somatic cells, and stem cell lines. This region was specifically unmethylated in the ovulated oocyte, where H1foo is expressed. 5-Aza-2'-deoxycytidine treatments and luciferase assays provided in vitro evidence that DNA methylation plays a role in repressing H1foo in nonexpressing cells. DNA methylation analyses of fetal germ cells revealed the T-DMR to be hypomethylated in female and male germ cells at Embryonic Day 9.5 (E9.5), whereas it was highly methylated in somatic cells at this stage. Intriguingly, the unmethylated status was continuously observed throughout oogenesis at E9.5, E12.5, E15.5, E18.5, in mature oocytes, and after...Continue Reading

References

Aug 8, 1997·Journal of Molecular Biology·Z F WangW F Marzluff
Apr 29, 1998·Development Genes and Evolution·T TadaM A Surani
Jan 26, 2000·Development, Growth & Differentiation·T YoshimizuY Matsui
Aug 10, 2000·Histochemistry and Cell Biology·B DrabentD Doenecke
Jan 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H E KasinskyJ Ausió
Sep 3, 2002·Mechanisms of Development·Petra HajkovaM Azim Surani
Sep 26, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kunio ShiotaNaka Hattori
Mar 27, 2003·Molecular Reproduction and Development·Shun SatoYasuhisa Matsui
Apr 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Raouf AlamiEric E Bouhassira
Feb 6, 2004·The Journal of Biological Chemistry·Naoko HattoriKunio Shiota
Feb 24, 2004·The Journal of Biological Chemistry·Koichiro NishinoKunio Shiota
May 14, 2004·Biology of Reproduction·Christopher B GeyerJohn R McCarrey
Jul 16, 2004·Molecular Biology and Evolution·José M Eirín-LópezJosefina Méndez
Sep 17, 2004·Biology of Reproduction·Mamoru TanakaEli Y Adashi
Feb 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Fei SongWilliam A Held
Mar 8, 2005·Molecular Cell·Michael BustinJae-Hwan Lim
Aug 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yukiko YamazakiRyuzo Yanagimachi
Aug 30, 2005·The Journal of Biological Chemistry·Sylvain DaujatRobert Schneider
May 24, 2006·Cytogenetic and Genome Research·J D LiebJ M Greally
Jun 10, 2006·Molecular Cell·Tracy K HaleRafael E Herrera
Oct 31, 2006·Nature Genetics·Florian EckhardtStephan Beck

❮ Previous
Next ❯

Citations

Oct 16, 2013·Reproductive Biomedicine Online·Michelle M Denomme, Mellissa R W Mann
Jul 16, 2010·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hiroki MuramotoKunio Shiota
Aug 8, 2012·Epigenetics : Official Journal of the DNA Methylation Society·Koji HayakawaKunio Shiota
Apr 30, 2015·Chromosoma·Salvador Pérez-MonteroFernando Azorín
Apr 10, 2010·Fertility and Sterility·Stephanie BeallJames Segars
Sep 21, 2010·Systems Biology in Reproductive Medicine·Rocío Melissa Rivera

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.